Seasonal Variation of the Major Allergen Fra a 1 in Strawberry Fruit

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Author(s)

Abstract

<p>Strawberry (<i>Fragaria</i> × <i>ananassa</i>) contains a major allergen, Fra a 1, which causes oral allergic syndrome. Fra a 1 is a PR-10 homolog that is regulated by environmental conditions. The allergenicity of fruit caused by Fra a 1 may depend on the genotype or growing conditions. We analyzed the <i>Fra a 1.01</i> transcript levels and Fra a 1.01 protein levels in strawberry fruits of several genotypes across all seasons. In the preliminary rough screening, we selected the line WH1 bearing white fruit and the red-fruited cultivar 'Akihime'. Under the same environmental conditions, there was no significant difference in Fra a 1.01 levels between the two cultivars over several months, suggesting that receptacle color was not indicative of allergenicity caused by Fra a 1.01. Fruits cultivated under the same environmental conditions should be used for comparisons of the allergenicity among genotypes. Both 'Akihime' and WH1 accumulated significantly higher levels of Fra a 1.01 protein in winter than in spring. We investigated the effects of irradiation and low temperature as environmental factors controlling the accumulation of Fra a 1.01 in winter. A shading treatment on fruit did not significantly affect Fra a 1.01 protein accumulation in strawberry fruits. Regarding variations over time, the Fra a 1.01 protein content was higher in fruits harvested at midnight in January than in those harvested at other times and in other months. These findings suggested that the Fra a 1.01 protein accumulates in response to environmental factors such as cold stress.</p>

Journal

  • The Horticulture Journal

    The Horticulture Journal 88(3), 354-363, 2019

    The Japanese Society for Horticultural Science

Codes

  • NII Article ID (NAID)
    130007682205
  • NII NACSIS-CAT ID (NCID)
    AA12708073
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    2189-0102
  • NDL Article ID
    030123963
  • NDL Call No.
    Z78-A456
  • Data Source
    NDL  IR  J-STAGE 
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