Effect of Resistant Starch on the Gut Microbiota and Its Metabolites in Patients with Coronary Artery Disease

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Author(s)

    • Yoshida Naofumi
    • Division of Cardiovascular Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine
    • Kondo Akihiko
    • Graduate School of Science, Technology and Innovation, Kobe University|RIKEN Center for Sustainable Resource Science, Yokohama, Japan
    • Sasaki Kengo
    • Graduate School of Science, Technology and Innovation, Kobe University
    • Sasaki Daisuke
    • Graduate School of Science, Technology and Innovation, Kobe University
    • Yamashita Tomoya
    • Division of Cardiovascular Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine
    • Hayashi Tomohiro
    • Division of Cardiovascular Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine
    • Tabata Tokiko
    • Division of Cardiovascular Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine
    • Osawa Ro
    • Research Center for Food Safety and Security, Graduate School of Agricultural Science, Kobe University|Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University
    • Hirata Ken-ichi
    • Division of Cardiovascular Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine

Abstract

<p><b>Aim:</b> <i>Bacteroides vulgatus</i> and <i>B. dorei</i> have a protective effect against atherosclerosis, suggesting that expansion of these species in the gut microbiota could help patients with coronary artery disease (CAD). This study aimed to investigate the effect of resistant starch (RS) on the gut microbiota and its metabolites in fecal sample cultures from patients with CAD and individuals without CAD, using a single-batch fermentation system.</p><p><b>Methods:</b> Fecal samples from 11 patients with CAD and 10 individuals without CAD were fermented for 30 h with or without RS in the Kobe University Human Intestinal Microbiota Model (KUHIMM). Gut microbiota and the abundance of <i>B. vulgatus</i> and <i>B. dorei</i> were analyzed using 16S ribosomal ribonucleic acid (rRNA) gene sequencing and the quantitative polymerase chain reaction. Short-chain fatty acids were analyzed using high-performance liquid chromatography.</p><p><b>Results:</b> Gut microbial analysis showed significantly lower levels of <i>B. vulgatus</i> and <i>B. dorei</i> in the original fecal samples from patients with CAD, which was simulated after 30 h of fermentation in the KUHIMM. Although RS significantly increased the absolute numbers of <i>B. vulgatus</i> and <i>B. dorei</i>, and butyrate levels in CAD fecal sample cultures, the numbers varied among each patient.</p><p><b>Conclusions:</b> The effect of RS on gut microbiota and its metabolites in the KUHIMM varied between CAD and non-CAD fecal sample cultures. The KUHIMM may be useful for preclinical evaluations of the effects of RS on the gut microbiota and its metabolites.</p>

Journal

  • Journal of Atherosclerosis and Thrombosis

    Journal of Atherosclerosis and Thrombosis 26(8), 705-719, 2019

    Japan Atherosclerosis Society

Codes

  • NII Article ID (NAID)
    130007686516
  • NII NACSIS-CAT ID (NCID)
    AA12022527
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    1340-3478
  • Data Source
    IR  J-STAGE 
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