Nitrous Acid and Diethyl Sulfate, as Chemical Mutagenic Agents, to Improve the Biomass, Metabolites, Enzyme Activity, and Antioxidant Activity of Wild <i>Phellinus igniarius</i>

• Guo Jinjing

  College of Pharmacy, Ningxia Medical University, Ningxia Engineering and Technology Research Center of Modern Hui Medicine, Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education at Ningxia Medical University

• Liu Cheng

  Medical Scientific and technologic Center, Ningxia Medical University

• Zhang Caifang

  College of Pharmacy, Ningxia Medical University, Ningxia Engineering and Technology Research Center of Modern Hui Medicine, Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education at Ningxia Medical University

• Wang Jing

  College of Pharmacy, Ningxia Medical University, Ningxia Engineering and Technology Research Center of Modern Hui Medicine, Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education at Ningxia Medical University

• Wang Sa

  College of Pharmacy, Ningxia Medical University, Ningxia Engineering and Technology Research Center of Modern Hui Medicine, Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education at Ningxia Medical University

• Liu Xiaoxi

  College of Pharmacy, Ningxia Medical University, Ningxia Engineering and Technology Research Center of Modern Hui Medicine, Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education at Ningxia Medical University

• Gao Xiaojuan

  College of Pharmacy, Ningxia Medical University, Ningxia Engineering and Technology Research Center of Modern Hui Medicine, Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education at Ningxia Medical University

• Wu Xiuli

  College of Pharmacy, Ningxia Medical University, Ningxia Engineering and Technology Research Center of Modern Hui Medicine, Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education at Ningxia Medical University

抄録

Various secondary metabolites with antioxidative effects have been reported to exist in Phellinus igniarius. However, the trace quantity of these metabolites in normal fermentation conditions deeply limits its medical values and potent clinical application. Some elicitors such as bioinducer and chemical inducer can improve the productivity of secondary metabolites which have certain biological activities in plants and fungus. In this study, nitrous acid (NA) and diethyl sulfate (DES), as potent killing and mutagenic chemical agents, to treat P. igniarius and enhance the metabolites contents. The variation of biomass, metabolite production, enzyme activity, and antioxidant efficacy of two mutant strains were investigated. The mutant strains exhibited better viability and produced more secondary metabolites. Altogether, these results indicate that chemical mutagenesis is an effective strategy to obtain high-activity mutant strains of P. igniarius with high yields of total flavonoids and total polysaccharides, the main constituents with antitumor and antioxidant activities.

収録刊行物

• Food Science and Technology Research

  Food Science and Technology Research 25 (3), 459-466, 2019

  公益社団法人 日本食品科学工学会

キーワード

• chemical mutagens
• <i>Phellinus igniarius</i>
• antioxidant activity
• secondary metabolites

詳細情報

• CRID: 1390282763133474304
• NII論文ID: 130007690041
• DOI: 10.3136/fstr.25.459
• ISSN: 18813984
• ISSN: 13446606
• Web Site: https://www.jstage.jst.go.jp/article/fstr/25/3/25_459/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可