Germination properties of spores on entomopathogenic microsporidia, <i>Trachipleistophora haruka</i> isolated from <i>Spodoptera litura</i>

• Nakamura Haruka

  Laboratory of Applied Entomology, Department of Agricultural Bioscience, College of Bioresource Sciences, Nihon University

• Hatakeyama Yoshinori

  Laboratory of Applied Entomology, Department of Agricultural Bioscience, College of Bioresource Sciences, Nihon University

• Arai Reina

  Laboratory of Applied Entomology, Department of Agricultural Bioscience, College of Bioresource Sciences, Nihon University

• Imura Yuji

  Laboratory of Applied Entomology, Department of Agricultural Bioscience, College of Bioresource Sciences, Nihon University

• Takahashi Moe

  Laboratory of Applied Entomology, Department of Agricultural Bioscience, College of Bioresource Sciences, Nihon University

• Iwano Hidetoshi

  Laboratory of Applied Entomology, Department of Agricultural Bioscience, College of Bioresource Sciences, Nihon University

Bibliographic Information

Other Title

• Germination properties of spores on entomopathogenic microsporidia, Trachipleistophora haruka isolated from Spodoptera litura

Abstract

 In this paper, we report on the specific spore germination conditions of the microsporidia Trachipleistophora haruka (Old name Trachipleistophora sp. OSL-2012-10 was reported by Shigano et al. (2015)) isolated from the common cutworm, Spodoptera litura from the Ogasawara Islands, Japan. We analyzed germination rates in varying salt conditions and found that alkaline stress was required for spore germination. Nosema bombycis NIS-001 of the experiment control germinated regardless of temperature only by treatment of 0.1 M KOH or 0.1 M KCl+3% (w/v) H₂O₂. However, Trachipleistophora haruka treatment by 0.85% NaCl solution at 25°C showed germination behavior similar to treatment of 0.1 M KOH and 0.1 M KCl+3% (w/v) H₂O₂. The results indicated that this microsporidia requires sodium or potassium ions to germinate.

Journal

• Journal of Insect Biotechnology and Sericology

  Journal of Insect Biotechnology and Sericology 88 (2), 2_027-2_030, 2019

  The Japanese Society of Sericultural Science

Keywords

• microsporidia
• spore hatch
• Trachipleistophora

Details

• CRID: 1390564238111834880
• NII Article ID: 130007692026
• NII Article ID: 40021999519
• NII Book ID: AA11558849
• DOI: 10.11416/jibs.88.2_027
• ISSN: 18847978
• ISSN: 13468073
• NDL BIB ID: 029840468
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I029840468
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • CiNii Articles
• Abstract License Flag: Disallowed