Modulation of α_vβ₃ Integrin via Transactivation of β₃ Integrin Gene on Murine Bone Marrow Macrophages by 1,25(OH)₂D₃, Retinoic Acid and Interleukin-4

• Kitazawa Sohei

  Department of Molecular Pathology, Ehime University Graduate School of Medicine

• Haraguchi Ryuma

  Department of Molecular Pathology, Ehime University Graduate School of Medicine

• Kohara Yukihiro

  Department of Molecular Pathology, Ehime University Graduate School of Medicine

• Kitazawa Riko

  Department of Molecular Pathology, Ehime University Graduate School of Medicine Division of Diagnostic Pathology, Ehime University Hospital

Abstract

The interleukin (IL)-4, 1,25(OH)₂D₃ and retinoic acid, increase surface expression of functional integrin α_vβ₃ on murine osteoclast precursors. All three agonists stimulate transcription of the β₃ gene, leading to increased steady-state levels of mRNA this protein. By contrast, mRNA levels of α_v remain unchanged. In each instance, the increase in the surface expression of the integrin results in increased migration of the cells onto an α_vβ₃ substrate. Because β₃ subunit, except platelet where β₃ subunit conform a dimer with α_IIb, associates solely with α_v subunit monogamously, while promiscuous α_v subunit combines with various subunit, our present data support the idea that the β₃ subunit governs the surface-expressed functional integrin complex.

Journal

• ACTA HISTOCHEMICA ET CYTOCHEMICA

  ACTA HISTOCHEMICA ET CYTOCHEMICA 52 (4), 77-83, 2019-08-30

  JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

Keywords

• β₃ integrin
• 1,25(OH)₂D₃
• retinoic acid
• transactivation
• <i>in situ</i> hybridization

Details

• CRID: 1390001288160831488
• NII Article ID: 130007696918
• DOI: 10.1267/ahc.19015
• ISSN: 13475800
• ISSN: 00445991
• Web Site: https://www.jstage.jst.go.jp/article/ahc/52/4/52_19015/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2020205272
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed