An immunohistochemical study on testicular steroidogenesis in the Sunda porcupine (<i>Hystrix javanica</i>)
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- NURLIANI Anni
- Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan Department of Biology, Faculty of Mathematics and Natural Sciences, Lambung Mangkurat University, South Kalimantan 70714, Indonesia
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- SASAKI Motoki
- Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan
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- BUDIPITOJO Teguh
- Department of Anatomy, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta 55281, Indonesia
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- TSUBOTA Toshio
- Laboratory of Wildlife Biology and Medicine, Department of Environmental Veterinary Science, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido 060-0818, Japan
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- SUZUKI Masatsugu
- United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan Laboratory of Zoo and Wildlife Medicine, Faculty of Applied Biological Science, Gifu University, Gifu 501-1193, Japan
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- KITAMURA Nobuo
- Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan
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Abstract
<p>In the testes of the Sunda porcupine (Hystrix javanica), the expression of the steroidogenic acute regulatory protein (StAR) and steroidogenic enzymes, such as cytochrome P450 side chain cleavage (P450scc), 3β-hydroxysteroid dehydrogenase (3β-HSD), cytochrome P450 17α-hydroxylase (P450c17) and cytochrome P450 aromatase (P450arom), was immunohistochemically examined to clarify the location of steroidogenesis. In this study, complete spermatogenesis (spermiogenesis) was observed in the testes of the examined Sunda porcupine, and spermatozoa of the Sunda porcupine had a spatulate sperm head unlike that of rats and mice which has an apical hook. On immunostaining of StAR, P450scc, 3β-HSD, P450c17 and P450arom, immunoreactivity for all proteins was only detected in the Leydig cells and not observed within the seminiferous tubules, suggesting that the Leydig cells can synthesize both androgen and estrogen from cholesterol in the Sunda porcupine testes.</p>
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 81 (9), 1285-1290, 2019
JAPANESE SOCIETY OF VETERINARY SCIENCE