Comparison of glucose tolerance between wild-type mice and mice with double knockout of neuromedin U and neuromedin S

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Author(s)

    • ENSHO Takuya
    • Department of Veterinary Physiology, Faculty of Agriculture, University of Miyazaki, 1-1, Gakuenkibanadainishi, Miyazaki-shi, Miyazaki 889-2192, Japan
    • MARUYAMA Keisuke
    • Department of Veterinary Physiology, Faculty of Agriculture, University of Miyazaki, 1-1, Gakuenkibanadainishi, Miyazaki-shi, Miyazaki 889-2192, Japan
    • QATTALI Abdul Wahid
    • Department of Veterinary Physiology, Faculty of Agriculture, University of Miyazaki, 1-1, Gakuenkibanadainishi, Miyazaki-shi, Miyazaki 889-2192, Japan
    • YASUDA Masahiro
    • Department of Veterinary Anatomy, Faculty of Agriculture, University of Miyazaki, 1-1, Gakuenkibanadainishi, Miyazaki-shi, Miyazaki 889-2192, Japan
    • UEMURA Ryoko
    • Department of Veterinary Domestic animal Hygienics, Faculty of Agriculture, 1-1, Gakuenkibanadainishi, Miyazaki-shi, Miyazaki 889-2192, Japan
    • MURAKAMI Noboru
    • Department of Veterinary Physiology, Faculty of Agriculture, University of Miyazaki, 1-1, Gakuenkibanadainishi, Miyazaki-shi, Miyazaki 889-2192, Japan
    • NAKAHARA Keiko
    • Department of Veterinary Physiology, Faculty of Agriculture, University of Miyazaki, 1-1, Gakuenkibanadainishi, Miyazaki-shi, Miyazaki 889-2192, Japan

Abstract

<p>Recently, it has been proposed that neuromedin U (NMU) is "decretin", which suppresses insulin secretion from the pancreas <i>in vitro</i>. Here we examined the possible involvement of NMU in insulin secretion <i>in vivo</i> by comparing the plasma glucose and insulin levels of wild-type mice with those of double knockout (D-KO) of the NMU and neuromedin S (NMS) genes, as NMS binds to the neuromedin U receptor. If NMU is, in fact, "decretin", which inhibits insulin secretion from the pancreas, then NMU-deficient mice might result in higher plasma insulin levels than is the case in wild-type mice, or injection of NMU lead to suppression of plasma insulin level. In this study, we found that the fasting plasma level of insulin was not increased in D-KO mice. Glucose tolerance tests revealed no significant difference in plasma insulin levels between wild-type mice and D-KO mice under non-fasting conditions. After peripheral injection of NMU, plasma glucose and insulin levels did not show any significant changes in either wild-type or D-KO mice. Glucose tolerance testing after 3 weeks of high fat feeding revealed no significant difference in plasma insulin levels during 60 min after glucose injection between wild-type and D-KO mice. These results suggest that even if NMU is a decretin candidate, its physiological involvement in suppression of insulin secretion may be very minor <i>in vivo</i>.</p>

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 81(9), 1305-1312, 2019

    JAPANESE SOCIETY OF VETERINARY SCIENCE

Codes

  • NII Article ID (NAID)
    130007708861
  • Text Lang
    ENG
  • ISSN
    0916-7250
  • Data Source
    J-STAGE 
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