バイオイメージング:超解像顕微鏡(STED)  [in Japanese] Bioimaging:Super-resolution microscope, stimulated emission depletion (STED)  [in Japanese]

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Abstract

<p>誘導放出制御(STED)顕微鏡は,数多く提案されている超解像蛍光顕微鏡の中でも,蛍光スポット(励起状態の空間)自体を回折限界以下に制限しながら走査して測定するという特徴をもつことから,バイオイメージングだけではなく,さらに一歩進んだ実験も行える可能性を秘めています.本稿では,STED顕微鏡を構築する際のさまざまなコツについて解説します.</p>

<p>Stimulated emission depletion (STED) microscopy is one of the fluorescence microscopy techniques, which enables sub-diffraction imaging of biological samples. The principle of the STED microscope and the role of optical components are explained. The alignment procedure and sample preparations are also discussed, and examples of STED images of fluorescent beads and actin filaments are demonstrated. Perspectives of the application of STED microscopy are also discussed.</p>

Journal

  • Oyo Buturi

    Oyo Buturi 87(10), 769-773, 2018

    The Japan Society of Applied Physics

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