Influence of Temperature on the Anti-allergic Activity of Fucoidan Extracted from <i>Saccharina japonica</i>

• Morita Atsuko

  Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University

• Tanino Yuka

  Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University Industrial Technology Center of Okayama Prefecture

• Ojima Takao

  Faculty of Fisheries Science, Hokkaido University

• Mizuno Masashi

  Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University

Abstract

It has been ascertained in our laboratory that fucoidan, a polysaccharide contained in Saccharina japonica, shows anti-allergic activity through galectin 9 secretion in blood. A crude fucoidan fraction was chromatographically fractionated into three fractions using a Toyopearl-DEAE 650 M column in stepwise elusion with 0, 0.5 and 1.0 M NaCl in 0.05 M Tris-HCl buffer (pH 7.4). Each fraction was assessed using the passive cutaneous anaphylaxis (PCA) reaction. The non-absorbed fraction of the three fractions suppressed PCA, whereas the fractions eluted with 0.5 M and 1.0 M NaCl did not. Moreover, it was discovered that heat treatment of fucoidan at 50 °C for 10 min abolished its anti-allergic activity in the PCA reaction. Using DEAE chromatography, it was demonstrated that heat-treatment of the crude fucoidan fraction decreased the non-absorbed fraction, which possessed the anti-allergic activity, and increased the two fractions eluted with 0.5 M and 1.0 M NaCl. This clearly revealed the importance of temperature in maintaining the anti-allergic activity of fucoidan.

Journal

• Food Science and Technology Research

  Food Science and Technology Research 25 (4), 607-611, 2019

  Japanese Society for Food Science and Technology

Keywords

• Anti-allergic activity
• fucoidan
• polysaccharide
• passive cutaneous anaphylaxis
• <i>Saccharina japonica</i>

Details

• CRID: 1390282752332067456
• NII Article ID: 130007715857
• NII Book ID: AA11320122
• DOI: 10.3136/fstr.25.607
• ISSN: 18813984
• ISSN: 13446606
• HANDLE: 20.500.14094/90006691
• Web Site: https://www.jstage.jst.go.jp/article/fstr/25/4/25_607/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed