Present status of large-scale culture methods of pluripotent stem cell : Differentiation of human iPS cells with dialysis suspension culture

DOI
  • Choi Hyunjin
    Department of Bioengineering, School of Engineering, The University of Tokyo
  • Shinohara Marie
    Department of Chemical Systems Engineering, School of Engineering, The University of Tokyo
  • Sakai Yasuyuki
    Department of Chemical Systems Engineering, Department of Bioengineering, School of Engineering, The University of Tokyo

Bibliographic Information

Other Title
  • 多能性幹細胞の大量培養法の現状：透析浮遊培養を用いたヒトiPS細胞の分化誘導

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Abstract

<p>Pluripotent stem cells (PSCs) are expected to be a promising cell source in the field of regenerative medicine and cell-based drug screening because of their high self-renewal capability while preserving pluripotency. To realize these applications, large-scale and efficient cell production methodologies are highly required. In this short review, we introduced typical suspension culture methods with their advantages and disadvantages. In addition, we explained our latest trials concerning pancreatic β cells differentiation of human induced pluripotent stem cells (hiPSCs) using suspension culture system with dialysis operations.</p>

Journal

  • Organ Biology

    Organ Biology 26 (2), 175-184, 2019

    The Japan Society for Organ Preservation and Biology

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