生体深部の高解像度イメージング技術  [in Japanese] Development of <i>in vivo</i> two-photon imaging with higher resolution in deeper region of the mouse brain  [in Japanese]

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Author(s)

    • 川上 良介 KAWAKAMI Ryosuke
    • 北海道大学 電子科学研究所 光細胞生理研究分野 Laboratory of molecular and cellular biophysics, Research Institute for Electronic Science, Hokkaido University
    • 根本 知己 NEMOTO Tomomi
    • 北海道大学 電子科学研究所 光細胞生理研究分野 Laboratory of molecular and cellular biophysics, Research Institute for Electronic Science, Hokkaido University

Abstract

<p>光技術を用いたイメージング技術はバイオ・物性研究に欠かせないツールとなってきている.多光子励起顕微鏡法による<i>in vivo</i>イメージングは近年の脳機能研究の発展に多大な貢献をしてきている.脳は散乱体であり,通常ではほんの数百µmの深さしか可視化できない.我々は新規高出力半導体レーザー光源および高感度蛍光検出器を組み合わせることでマウス生体脳深部(1.5mm)に位置する海馬歯状回を非侵襲で高解像度イメージングすることに成功した.</p>

<p>Imaging techniques using optical technology are an indispensable tool for biological research. <i>in vivo</i> imaging by using two-photon microscopy has significantly contributed to the research of brain functions. The brain is a scattering body and only a depth of several hundred micrometers can be visualized with the normal two-photon microscopy. We have succeeded in high-resolution imaging of the hippocampal dentate gyrus that is located deep in the brain (1.5 millimeters) in a living mouse with a non-invasive approach by combining a novel high-peak power semiconductor LD light source and a high sensitivity fluorescence GaAsP- Non-Descanned detector.</p>

Journal

  • Oyo Buturi

    Oyo Buturi 84(10), 913-917, 2015

    The Japan Society of Applied Physics

Codes

  • NII Article ID (NAID)
    130007718660
  • NII NACSIS-CAT ID (NCID)
    AN00026679
  • Text Lang
    JPN
  • ISSN
    0369-8009
  • NDL Article ID
    026792471
  • NDL Call No.
    Z15-243
  • Data Source
    NDL  J-STAGE 
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