Birth of first foals through embryo transfer from Hokkaido native pony after artificial insemination using frozen semen

• HANNAN M A

  Obihiro University of Agriculture and Veterinary Medicine

• HANEDA Shingo

  Obihiro University of Agriculture and Veterinary Medicine

• MURATA Kaishi

  Obihiro University of Agriculture and Veterinary Medicine

• TAKEUCHI Shiori

  Obihiro University of Agriculture and Veterinary Medicine

• SAITOH Tomoko

  Obihiro University of Agriculture and Veterinary Medicine

• CHEONG Soon Hon

  Cornell University

• NAMBO Yasuo

  Obihiro University of Agriculture and Veterinary Medicine Gifu University

抄録

Embryo transfer technology allows the donor mare to potentially produce multiple foals in a year. In Japan, there has been no report of foal born through embryo transfer after artificial insemination using frozen semen. The aims were to develop a special riding crossbred horse and multiply its number through embryo transfer technology. In both donor and recipient mares, luteolysis was induced by administration of 0.1 mg PGF2α analogue to synchronize the onset of estrus, and ovulation was induced by administering 2000 IU of hCG. Frozen semen from an Irrish Connemara pony was used to breed a Hokkaido native pony by deep-horn artificial insemination (dose, 400 × 10⁶ sperms). A non-surgical transcervical procedure was used to collect embryo from the uterus of the donor mare at day 7 post-ovulation and transferred fresh into the uterus of recipient mare. Weekly blood sampling for monitoring pregnancy associated hormones and transrectal ultrasound for observing fetal development were done throughout the pregnancy. Four embryos were collected from a single donor mare and were transferred to recipients in spring 2018. Three out of 4 recipient mares (75 %) established successful pregnancy and 2 of them have already delivered a healthy foal each in late April 2019, another recipient is expected to give birth in around 1 month. A normal progesterone and equine chorionic gonadotropin hormone profile were observed during the gestation in all recipient mares. This are the first foals produced through embryo transfer in Japan after artificial insemination using frozen semen. We are expecting that this new crossbred (Connemara pony x Hokkaido native pony) would be an ideal riding breed for disable people.

収録刊行物

• 日本繁殖生物学会　講演要旨集

  日本繁殖生物学会　講演要旨集 112 (0), OR2-24-OR2-24, 2019

  公益社団法人 日本繁殖生物学会

詳細情報

• CRID: 1390001277359163264
• NII論文ID: 130007719260
• DOI: 10.14882/jrds.112.0_or2-24
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可