Micro-CT imaging analysis for the effects of ibandronate and eldecalcitol on secondary osteoporosis and arthritis in adjuvant-induced arthritis rats
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- ONO Yuichi
- Department of Orthopedic Surgery, Akita University Graduate School of Medicine
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- MIYAKOSHI Naohisa
- Department of Orthopedic Surgery, Akita University Graduate School of Medicine
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- KASUKAWA Yuji
- Department of Orthopedic Surgery, Akita University Graduate School of Medicine
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- IMAI Yuuki
- Division of Integrative Pathophysiology, Proteo-Science Center, Ehime University Graduate School of Medicine
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- NAGASAWA Hiroyuki
- Department of Orthopedic Surgery, Akita University Graduate School of Medicine
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- TSUCHIE Hiroyuki
- Department of Orthopedic Surgery, Akita University Graduate School of Medicine
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- AKAGAWA Manabu
- Department of Orthopedic Surgery, Akita University Graduate School of Medicine
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- NAGAHATA Itsuki
- Department of Orthopedic Surgery, Akita University Graduate School of Medicine
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- YUASA Yusuke
- Department of Orthopedic Surgery, Akita University Graduate School of Medicine
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- SATO Chiaki
- Department of Orthopedic Surgery, Akita University Graduate School of Medicine
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- KAWATANI Masahito
- Department of Neurophysiology, Akita University Graduate School of Medicine
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- SHIMADA Yoichi
- Department of Orthopedic Surgery, Akita University Graduate School of Medicine
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Abstract
<p>We investigated the effects of ibandronate, a bisphosphonate; eldecalcitol, an active vitamin D3 analogue; and combination treatment with both agents on secondary osteoporosis and arthritis using rats with adjuvant-induced arthritis. Arthritis was induced in 8-week-old male Lewis rats. Rats were randomized into four treatment groups and an untreated normal control group: ibandronate, eldecalcitol, ibandronate + eldecalcitol, vehicle, and control. Paw thickness was measured to evaluate arthritis. Joint destruction was evaluated histomorphometrically by the ankle joint stained with Fast Green and safranin O. The femur and lumbar spine were scanned using dual-energy X-ray absorptiometry, and the distal femur was scanned using micro-computed tomography for bone mineral density (BMD) and trabecular microstructural evaluations. Ibandronate and/or eldecalcitol increased BMD in both the lumbar vertebrae and femur and improved several microstructural parameters (bone volume/total volume, structure model index, trabecular number, and trabecular separation of the distal femur). In addition, there was an additive effect of combination treatment compared with single treatments for most trabecular parameters, including BMD and bone volume. However, ibandronate and/or eldecalcitol did not inhibit arthritis and joint destruction. Combination treatment with ibandronate and eldecalcitol may be effective for secondary osteoporosis associated with arthritis.</p>
Journal
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- Biomedical Research
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Biomedical Research 40 (5), 197-205, 2019-10-01
Biomedical Research Press