Supplemental study on 2’, 3’-Cyclic Nucleotide 3’-Phosphodiesterase (CNPase) activity in developing rat spinal cord lesions induced by hexachlorophene and cuprizone

• KANNO Takeshi

  Pathology Department, Kashima Laboratory, Nonclinical Research Center, LSI Medience Corporation, 14-1 Sunayama, Kamisu, Ibaraki 314-0255, Japan

• KUROTAKI Tetsuro

  Pathology Department, Kashima Laboratory, Nonclinical Research Center, LSI Medience Corporation, 14-1 Sunayama, Kamisu, Ibaraki 314-0255, Japan

• YAMADA Naoaki

  Pathology Department, Kashima Laboratory, Nonclinical Research Center, LSI Medience Corporation, 14-1 Sunayama, Kamisu, Ibaraki 314-0255, Japan

• TOMONARI Yuki

  Pathology Department, Kashima Laboratory, Nonclinical Research Center, LSI Medience Corporation, 14-1 Sunayama, Kamisu, Ibaraki 314-0255, Japan

• SATO Junko

  Pathology Department, Kashima Laboratory, Nonclinical Research Center, LSI Medience Corporation, 14-1 Sunayama, Kamisu, Ibaraki 314-0255, Japan

• TSUCHITANI Minoru

  Pathology Department, Kashima Laboratory, Nonclinical Research Center, LSI Medience Corporation, 14-1 Sunayama, Kamisu, Ibaraki 314-0255, Japan

• KOBAYASHI Yoshiyasu

  School of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan

Abstract

In a previous study, we showed that 2’, 3’-Cyclic Nucleotide 3’-Phosphodiesterase (CNPase) expression is induced in different temporal patterns in the cerebrum, cerebellum and medulla oblongata of hexachlorophene (HCP) and cuprizone (CPZ) treated rats. Here, we additionally examined the histopathological changes and CNPase expression in the spinal cord to clarify the reproducibility of different temporal patterns of CNPase expression in the spinal cord showing low degree or lack of spongy changes. Spongy changes were observed in HCP-treated rats, but not in CPZ-treated rats. Immunohistochemistry showed that intense expression of CNPase was not induced following HCP or CPZ treatment. Our data reveal that expression intensity of CNPase may be dependent on the degree of HCP- and CPZ-induced damage of the myelin sheath.

Journal

• Journal of Veterinary Medical Science

  Journal of Veterinary Medical Science 81 (9), 1368-1372, 2019

  JAPANESE SOCIETY OF VETERINARY SCIENCE

Keywords

• 2’, 3’-Cyclic Nucleotide 3’-Phosphodiesterase
• cuprizone
• hexachlorophene
• rat
• spinal cord

Details

• CRID: 1390564227320558336
• NII Article ID: 130007725684
• DOI: 10.1292/jvms.19-0096
• ISSN: 13477439
• ISSN: 09167250
• PubMed: 31447458
• Web Site: https://www.jstage.jst.go.jp/article/jvms/81/9/81_19-0096/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2020125759
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed