Identification of Intermediates in Peroxidase Catalytic Cycle of a DNAzyme Possessing Heme

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    • Yamamoto Yasuhiko
    • Department of Chemistry, University of Tsukuba|Tsukuba Research Center for Energy Materials Science (TREMS), University of Tsukuba|Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba


<p>Heme in the ferric state (heme(Fe<sup>3+</sup>)) binds to G-quadruplex DNAs to form stable complexes that exhibit enhanced peroxidase activities. The complexes are considered DNAzymes possessing heme as a prosthetic group (heme-DNAzymes), and have been extensively investigated as promising catalysts for a variety of applications. On ESR and stopped-flow measurements, an iron(IV)oxo porphyrin π-cation radical known as Compound I was detected in reaction mixtures of heme-DNAzymes and hydrogen peroxide. This finding not only resolved the long-standing issue of the mechanism underlying the enhancement of the peroxidase activity of heme(Fe<sup>3+</sup>) in the scaffold of a G-quadruplex DNA, but also provided new insights as to the design of novel heme-DNAzymes.</p>


  • Bulletin of the Chemical Society of Japan

    Bulletin of the Chemical Society of Japan 92(10), 1729-1736, 2019

    The Chemical Society of Japan


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