Detection of canine <i>Schistosoma japonicum</i> infection using recombinant thioredoxin peroxidase-1 and tandem repeat proteins
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- ANGELES Jose Ma. M.
- Department of Parasitology, College of Public Health, University of the Philippines, Manila 1000, Philippines National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
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- GOTO Yasuyuki
- Laboratory of Molecular Immunology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan
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- KIRINOKI Masashi
- Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi 321-0293, Japan
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- LEONARDO Lydia R.
- Department of Parasitology, College of Public Health, University of the Philippines, Manila 1000, Philippines
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- MOENDEG Kharleezelle J.
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan Department of Biology, School of Science and Engineering, Ateneo de Manila University, Quezon City 1108, Philippines
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- YBAÑEZ Adrian P.
- College of Veterinary Medicine (Barili Campus) and Center for Vector-Borne and Protozoan Diseases (Main Campus), Cebu Technological University, Cebu City 6000, Philippines
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- RIVERA Pilarita T.
- Department of Parasitology, College of Public Health, University of the Philippines, Manila 1000, Philippines
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- VILLACORTE Elena A.
- Department of Parasitology, College of Public Health, University of the Philippines, Manila 1000, Philippines
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- INOUE Noboru
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
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- CHIGUSA Yuichi
- Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi 321-0293, Japan
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- KAWAZU Shin-ichiro
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
Bibliographic Information
- Other Title
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- Detection of canine Schistosoma japonicum infection using recombinant thioredoxin peroxidase-1 and tandem repeat proteins
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Abstract
<p>Humans and dogs live very close together and share various pathogens causing zoonotic parasitoses like schistosomiasis. A previous population genetics study done for schistosomes in the Philippines suggested that there is a high transmission level of Schistosoma japonicum among humans and dogs proving that the latter are important reservoirs for this zoonotic parasite. A more sensitive and specific test detecting schistosome infection in dogs will therefore strengthen the zoonotic surveillance, which might help in the possible elimination of this ancient disease. In this study, recombinant thioredoxin peroxidase-1 (SjTPx-1) and tandem repeat proteins (Sj1TR, Sj2TR, Sj4TR, Sj7TR) previously tested on human and water buffalo samples were used to assess its diagnostic applicability to dogs. Fifty-nine dog serum and stool samples were collected in the schistosomiasis-endemic municipalities of Calatrava, Negros Occidental and Catarman, Northern Samar in the Philippines and examined using the ELISA as compared to microscopy and fecal sample-based PCR. Samples positive for Babesia gibsoni and Dirofilaria immitis were also used to check for cross-reaction. Results showed that SjTPx-1 (80% sensitivity, 92.3% specificity) and Sj7TR (73.3% sensitivity, 92.3% specificity) have good potentials for diagnosing S. japonicum infection in dogs. These diagnostic antigens will therefore improve the surveillance in the transmission of the parasites from dogs to humans.</p>
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 81 (10), 1413-1418, 2019
JAPANESE SOCIETY OF VETERINARY SCIENCE