Determination of Polymorphisms in Pituitary Genes of the Native Afghani Naked Neck Chicken

• Ahmadi Sadequllah

  College of Agriculture, Ibaraki University Faculty of Animal Science, Afghanistan National Agricultural Sciences and Technology University (ANASTU)

• Takeda Misa

  College of Agriculture, Ibaraki University

• Ohkubo Takeshi

  College of Agriculture, Ibaraki University

Abstract

We investigated means to improve the production of the indigenous Naked Neck chicken in Afghanistan. Specifically, we analyzed single nucleotide polymorphisms (SNPs) in the prolactin (PRL) (24 bp indel), growth hormone (GH) (T185G), and pituitary specific transcript factor 1 (PIT-1) (intron 5) genes. Blood samples were collected from 52 birds and genomic DNA was extracted. Polymorphisms in the mentioned loci were analyzed by PCR, allele-specific PCR, and PCR-restriction fragment length polymorphism (RFLP) using TaqI and MspI endonucleases. Cloning followed by DNA sequencing was performed to ascertain the accuracy of the PCR-RFLP analysis for PIT-1.Two alleles were found for the PRL 24 bp indel, GH (T185G), and PIT-1/TaqI, with the following respective allelic frequencies: PRL-In 0.64 and PRL-Del 0.36, GH-T 0.91 and GH-G 0.09, and PIT-1-A 0.64 and PIT-1-B 0.36. Regarding the PIT-1/MspI polymorphism, three novel MspI recognition sites, as well as two reported MspI recognition sites, were detected in intron 5. Moreover, during sequence screening, two novel SNPs were found that generated restriction sites for MseI. Therefore, our results suggest that the PRL indel, GH T185G, and PIT-1/TaqI polymorphisms may be used as selection markers for Afghanistan Naked Neck chickens. Intron 5 of PIT-1 in the Afghani Naked Neck chicken was highly polymorphic compared to the reported Gallus gallus PIT-1 gene (GenBank accession no. NC_006088.4).

Journal

• The Journal of Poultry Science

  The Journal of Poultry Science 56 (4), 253-261, 2019

  Japan Poultry Science Association

Keywords

• genetic polymorphism
• Naked Neck
• <i>PIT-1</i>
• pituitary hormone

Details

• CRID: 1390845702303938176
• NII Article ID: 130007733294
• NII Book ID: AA11564513
• DOI: 10.2141/jpsa.0180087
• ISSN: 13490486
• ISSN: 13467395
• NDL BIB ID: 030030739
• Web Site: http://id.ndl.go.jp/bib/030030739
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030030739
• Web Site: https://www.jstage.jst.go.jp/article/jpsa/56/4/56_0180087/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed