Minimal Sustainability of Dedifferentiation by ROCK Inhibitor on Rat Nucleus Pulposus Cells In Vitro
-
- Nukaga Tadashi
- Department of Orthopaedic Surgery, Tokai University School of Medicine
-
- Sakai Daisuke
- Department of Orthopaedic Surgery, Tokai University School of Medicine
-
- Schol Jordy
- Department of Orthopaedic Surgery, Tokai University School of Medicine
-
- Suyama Kaori
- Department of Anatomy and Cellular Biology, Tokai University School of Medicine
-
- Nakai Tomoko
- Department of Orthopaedic Surgery, Tokai University School of Medicine
-
- Hiyama Akihiko
- Department of Orthopaedic Surgery, Tokai University School of Medicine
-
- Watanabe Masahiko
- Department of Orthopaedic Surgery, Tokai University School of Medicine
この論文をさがす
抄録
<p>Introduction: Intervertebral disc degeneration is strongly associated with low back pain. Cell transplantation has been extensively studied as a treatment option for intervertebral disc degeneration. It is often necessary to perform cell culture prior to cell transplantation; however, during cell expansion, the cells tend to dedifferentiate and lose their potency. Although the ability to suppress dedifferentiation by ROCK inhibitor (ROCKi) has recently been reported for chondrocytes, its effects on nucleus pulposus cells are still largely unknown.</p><p>Methods: Rat nucleus pulposus cells were cultured with or without the addition of ROCKi (Y-27632), and cell proliferation; CD24 positivity; expression of SOX9, COL2A1, Aggrecan, and COL1A1; and cell redifferentiation ability in pellet culture were evaluated.</p><p>Results: Although the addition of ROCKi tended to slightly increase the cell proliferative capacity, no significant differences were observed between treated and untreated conditions. The addition of ROCKi showed a trend of minimally increased COL2A1, ACAN, and SOX9 expression. Increases in COL1A1 expression was slightly suppressed by ROCKi. In pellet culture, strong increase in type II collagen deposition was observed by the addition of ROCKi. The addition of ROCKi did not significantly change the levels of CD24 positivity. The supplementation of ROCKi did not significantly enhance nucleus pulposus cell marker expression during monolayer expansion. However, ROCKi addition did result in an increased type II collagen deposition in 3D pellet culture.</p><p>Conclusions: Taken together, the results suggest a minimal effect by ROCKi on nucleus pulposus cell phenotype maintenance.</p>
収録刊行物
-
- Spine Surgery and Related Research
-
Spine Surgery and Related Research 3 (4), 385-391, 2019-10-27
一般社団法人 日本脊椎脊髄病学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390845702304643584
-
- NII論文ID
- 130007734403
-
- ISSN
- 2432261X
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- Crossref
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可