Production of Intergeneric Hybrids between <i>Argyranthemum frutescens</i> (L.) Sch. Bip. and <i>Rhodanthemum gayanum</i> (Cross. & Durieu) B.H. Wilcox, K. Bremer & Humphries Using Embryo Culture and Gene Markers for Discrimination

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Author(s)

    • Muto Takahiro
    • Graduate School of Horticulture, Chiba University|Seibu Agriculture and Forestry Office
    • Ando Masaya
    • Center for Environment, Health and Field Sciences, Chiba University
    • Katsuoka Hiroyuki
    • Shizuoka Research Institute of Agriculture and Forestry/Izu Agricultural Research Center
    • Inaba Zentaro
    • Shizuoka Research Institute of Agriculture and Forestry/Izu Agricultural Research Center

Abstract

<p>In this study, we performed intergeneric crosses between <i>Argyranthemum frutescens</i> (L.) Sch. Bip. and seven perennial species of closely related genera [<i>Artemisia absinthium</i> L., <i>Chrysanthemum lavandulifolium</i> (Fisch. ex Trautv.) Makino × <i>Chrysanthemum</i> × <i>morifolium</i> Ramat., <i>Dimorphotheca sinuata</i> DC., <i>Osteospermum ecklonis</i> (de Candolle) Norlindh, <i>Pericallis hybrida</i> B. Nord., <i>Rhodanthemum gayanum</i> (Cross. & Durieu) B.H. Wilcox, K. Bremer & Humphries., and <i>Rhodanthemum hosmariense</i> (Ball) B.H. Wilcox, K. Bremer & Humphries.]. Using an embryo culture technique to generate intergeneric hybrids, we produced two putative hybrids from the crosses between <i>A. frutescens</i> and <i>R. gayanum</i>. In the putative hybrid derived from the cross between <i>A. frutescens</i> 'Brilliant rouge' × <i>R. gayanum</i> 'Elf pink', its ligulate flower color was similar to the 'Brilliant rouge' seed parent while the composition and total amount of anthocyanidins and/or leucoanthocyanidins were different. In the putative hybrid derived from the cross between <i>A. frutescens</i> 'Sunday ripple' and <i>R. gayanum</i> 'African eyes', its ligulate flower color differed from those of the parents. The ligulate flowers of the parents were white and produced no anthocyanidins, whereas the putative hybrids had light pink ligulate flowers and produced three anthocyanidins pigments [pelargonidins (including pelargonidin and/or leucopelargonidin), cyanidins (including cyanidin and/or leucocyanidin, peonidin), and delphinidins (including delphinidin and/or leucodelphinidin, malvidin)]. In addition, the cleaved amplified polymorphic sequence (CAPS) markers (<i>Afl</i> II) developed in this study confirmed that the putative hybrids were intergeneric hybrids of <i>A. frutescens</i> × <i>R. gayanum</i>. Therefore, these CAPS selection markers can be used to determine whether plants resulting from crosses are hybrids.</p>

Journal

  • The Horticulture Journal

    The Horticulture Journal, 2020

    The Japanese Society for Horticultural Science

Codes

  • NII Article ID (NAID)
    130007739885
  • Text Lang
    ENG
  • ISSN
    2189-0102
  • Data Source
    J-STAGE 
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