Fluorous Derivatization Method for Selective Analysis of Curcumin with Liquid Chromatography-Tandem Mass Spectrometry
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- KAWASUE Shimba
- Faculty of Pharmaceutical Sciences, Fukuoka University
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- SAKAGUCHI Yohei
- Faculty of Pharmaceutical Sciences, Fukuoka University
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- YANO Ena
- Faculty of Pharmaceutical Sciences, Fukuoka University
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- HAYAMA Tadashi
- Faculty of Pharmaceutical Sciences, Fukuoka University
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- KOGA Reiko
- Faculty of Pharmaceutical Sciences, Fukuoka University
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- YOSHIDA Hideyuki
- Faculty of Pharmaceutical Sciences, Fukuoka University
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- NOHTA Hitoshi
- Faculty of Pharmaceutical Sciences, Fukuoka University
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Abstract
<p>Curcumin has been shown to be pharmacologically active in the prevention and treatment of various human diseases. In this study, we developed a fluorous derivatization method for selective analysis of curcumin with liquid chromatography (LC)tandem mass spectrometry (MS/MS). Curcumin was derivatized with the thiol-containing fluorous reagent (1H,1H,2H,2Hperfluoro-1-decanethiol) under mild conditions via Michael addition reaction, and the obtained derivative was introduced to a fluorous LC column (Fluofix-II 120E, 150 × 2.0 mm i.d., 5 μm, Wako). The selectively retained fluorous-derivatized curcumin on the column was also enabled highly sensitive detection with negative electrospray ionization MS/MS. Pretreatment of human serum sample was used protein precipitation with CH3CN. The calibration curve obtained by the present method showed good linearity (r2 = 0.9998) in the range of 7.4-442 ng/mL serum, and the limit of detection (S/N = 3) and limit of quantification (S/N = 10) were 1.8 ng/mL serum and 6.1 ng/mL serum, respectively. The present method was successfully applied to the analysis of curcumin in human serum sample.</p>
Journal
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- CHROMATOGRAPHY
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CHROMATOGRAPHY 40 (3), 115-119, 2019-10-20
The Society for Chromatographic Sciences
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Details 詳細情報について
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- CRID
- 1390001277381537536
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- NII Article ID
- 130007741845
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- NII Book ID
- AA1137755X
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- ISSN
- 13483315
- 13428284
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- NDL BIB ID
- 030072287
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed