Single Cell Receptor Analysis Aided by a Centrifugal Microfluidic Device for Immune Cells Profiling

• Chen Zhu

  Department of Applied Physics, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871 , Japan

• Wilfred Villariza Espulgar

  Department of Applied Physics, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871 , Japan

• Woosik Yoo

  WaferMasters Incorporated, 254 East Gish Road, San Jose, CA 95112 , USA

• Shohei Koyama

  Immunology Frontier Research Center, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871 , Japan

• Xiaoming Dou

  Institute of Photonics and Bio-medicine (IPBM), Graduate School of Science, East China University of Science and Technology (ECUST), 130 Meilong Road, Shanghai 200237, P. R. China

• Atsushi Kumanogoh

  Immunology Frontier Research Center, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871 , Japan

• Eiichi Tamiya

  Department of Applied Physics, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871 , Japan

• Hyota Takamatsu

  Immunology Frontier Research Center, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871 , Japan

• Masato Saito

  Department of Applied Physics, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871 , Japan

Abstract

Single cell analysis has been the main focus of studies among scientists in recent decades for its outstanding contribution to medical treatment. An alternative method has been developed using a centrifugal microfluidic device to trap single cells, conduct immunostaining, and measure the cell surface receptor fluorescence intensity. The ratio of the fluorescence intensity can be used to profile the cell differentiation and the archived images can be useful in further analysis of the obtained data points. This could provide information on the morphological condition and receptor protein distribution on the surfaceome of the cells. To demonstrate the utility of the device, THP-1 and Jurkat Cells were tested and profiled with CD3, CD13, and CD31 markers. The results show that the device has performance similar to Fluorescence-activated cell sorting method (FACS) using relatively small sample volume and low cell suspension concentration. The utility of this device can be proven in identifying new surface markers and insight into basic biology.

Journal

• Bulletin of the Chemical Society of Japan

  Bulletin of the Chemical Society of Japan 92 (11), 1834-1839, 2019-08-22

  Oxford University Press (OUP)

Keywords

• General Chemistry

Details

• CRID: 1360283694073853312
• NII Article ID: 130007745892
• DOI: 10.1246/bcsj.20190175
• ISSN: 13480634
• ISSN: 00092673
• Web Site: https://academic.oup.com/bcsj/article-pdf/92/11/1834/56299723/bcsj.20190175.pdf
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