Quantitative Bio-imaging of Gadolinium-157 in Tissues Through Laser-ablation ICP-MS for Neutron Capture Therapy

• KUBOTA AYANO

  Department of Epidemiology and Environmental Health, Juntendo University Faculty of Medicine

• MATSUKAWA TAKEHISA

  Department of Epidemiology and Environmental Health, Juntendo University Faculty of Medicine Department of Forensic Medicine, Juntendo University Faculty of Medicine

• YANAGIE HIRONOBU

  Department of Nuclear Engineering and Management, The University of Tokyo

• YANAGAWA MASASHI

  Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine

• HIRATA TAKAFUMI

  Geochemical Research Center, The University of Tokyo

• SHINOHARA ATSUKO

  Department of Epidemiology and Environmental Health, Juntendo University Faculty of Medicine Research Institute for Cultural Studies, Seisen University

• YOKOYAMA KAZUHITO

  Department of Epidemiology and Environmental Health, Juntendo University Faculty of Medicine Department of Epidemiology and Social Medicine, International University of Health and Welfare Graduate School of Public Health

抄録

Objective: Gadolinium-157 (¹⁵⁷Gd) has attracted interest for its use in the preparation containing metal elements of the neutron capture therapy (NCT), one of the radiation therapies. The study, however, has not developed because of the difficulty to quantify the concentration of ¹⁵⁷Gd in the tissue. Therefore, we established a quantitative imaging technique for ¹⁵⁷Gd in bio-tissue employing laser-ablation inductively coupled mass spectrometry (LA-ICP-MS). Materials and Methods: 4 female New Zealand white rabbits, which were inoculated with rabbit VX-2 cells participated in this study. ¹⁵⁷Gd in water-in-oil-in-water (WOW) emulsion was injected via the proper hepatic artery into the rabbits and, after 24 or 72 hours, the rabbits were killed, and the liver tissues were harvested. We also prepared 7 standard tissues which were mixed with gadoteridol solutions, the final amount of ¹⁵⁷Gd was 0, 4.4, 22, 44, 220, 440 and 660 μg/g, respectively. The harvested livers and standards were sectioned on a cryostat at 5 μm intervals and they were analyzed by LA-ICP-MS. Result: In an experiment on animal cancer tissue, ¹⁵⁷Gd was observed to accumulate around the cancer. Conclusion: The ¹⁵⁷Gd concentration in bio tissue can be quantitatively assessed through LA-ICP-MS imaging and it was expected to contribute the progress of NCT study.

収録刊行物

• 順天堂醫事雑誌

  順天堂醫事雑誌 65 (5), 461-467, 2019

  順天堂医学会

キーワード

• gadolinium
• laser-ablation inductively coupled mass spectrometry (LA-ICP-MS)
• neutron capture therapy (NCT)
• bio-imaging

詳細情報

• CRID: 1390564227351719040
• NII論文ID: 130007752696
• NII書誌ID: AA1262207X
• DOI: 10.14789/jmj.2019.65.jmj19-oa12
• ISSN: 21882126
• ISSN: 21879737
• NDL書誌ID: 030158038
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030158038
• Web Site: https://www.jstage.jst.go.jp/article/jmj/65/5/65_JMJ19-OA12/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2020317039
• 本文言語コード: en
• データソース種別:
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  • NDL
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  • CiNii Articles
  • KAKEN
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