Handmade leaf cutter for efficient and reliable ROS assay

• Desaki Yoshitake

  Department of Life Sciences, School of Agriculture, Meiji University Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science

• Shimada Hikaru

  Department of Life Sciences, School of Agriculture, Meiji University

• Takahashi Shohei

  Department of Life Sciences, School of Agriculture, Meiji University

• Sakurayama Chisa

  Department of Life Sciences, School of Agriculture, Meiji University

• Kawai Mika

  Department of Life Sciences, School of Agriculture, Meiji University

• Kaku Hanae

  Department of Life Sciences, School of Agriculture, Meiji University

• Shibuya Naoto

  Department of Life Sciences, School of Agriculture, Meiji University

Abstract

Reactive oxygen species generation is one of the most popular index of plant immune responses. Leaf disk assay has been commonly used for MAMP/elicitor-induced ROS analysis by many groups. However, the reproducibility of the leaf disk assay relies on the skills of the people engaged in the experiments and the experiment itself seems not suitable for some plant species, which had a tough leaf structure and lower penetration efficiency of MAMPs/elicitors. In this study, we prepared a handmade leaf cutter to cut out the leaf fragments with uniform size and slits. The use of such fragments obtained by the new leaf cutter as well as the increase of the number of leaf fragments for each experiment improved the reliability and reproducibility of the leaf disk assay. This cutter was also successfully applied to rice leaf disk assay, indicating the applicability to other plant spices.

Journal

• Plant Biotechnology

  Plant Biotechnology 36 (4), 275-278, 2019-12-25

  Japanese Society for Plant Biotechnology

Keywords

• defense response
• elicitor
• leaf disk
• MAMP
• ROS

Details

• CRID: 1390846609789052416
• NII Article ID: 130007776098
• NII Book ID: AA11250821
• DOI: 10.5511/plantbiotechnology.19.0921a
• ISSN: 13476114
• ISSN: 13424580
• NDL BIB ID: 030188488
• Web Site: http://id.ndl.go.jp/bib/030188488
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030188488
• Web Site: https://www.jstage.jst.go.jp/article/plantbiotechnology/36/4/36_19.0921a/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed