Isolation and Screening of Indigenous Plant Growth-promoting Rhizobacteria from Different Rice Cultivars in Afghanistan Soils

• Habibi Safiullah

  Faculty of Agriculture, Kabul University

• Djedidi Salem

  Institute of Agriculture, Tokyo University of Agriculture and Technology

• Ohkama-Ohtsu Naoko

  Institute of Agriculture, Tokyo University of Agriculture and Technology

• Sarhadi Wakil Ahmad

  Faculty of Agriculture, Kabul University

• Kojima Katsuhiro

  Institute of Agriculture, Tokyo University of Agriculture and Technology

• Rallos Roland V.

  Nuclear Research Institute

• Ramirez Maria Daniela Artigas

  Institute of Agriculture, Tokyo University of Agriculture and Technology

• Yamaya Hiroko

  Institute of Agriculture, Tokyo University of Agriculture and Technology

• Sekimoto Hitoshi

  Faculty of Agriculture, Utsunomiya University

• Yokoyama Tadashi

  Institute of Agriculture, Tokyo University of Agriculture and Technology

抄録

To develop biofertilizers for rice in Afghanistan, 98 plant growth-promoting rhizobacteria were isolated from rice plants and their morphological and physiological characteristics, such as indole-3-acetic acid production, acetylene reduction, phosphate and potassium solubilization, and siderophore production, were evaluated. The genetic diversity of these bacteria was also analyzed based on 16S rRNA gene sequences. Of 98 bacteria, 89.7% produced IAA, 54.0% exhibited nitrogenase activity, and 40% showed phosphate solubilization and siderophore production. Some isolates assigned to Pseudomonas (brassicacearum, chengduensis, plecoglossicida, resinovorans, and straminea) formed a relationship with rice, and P. resinovorans and P. straminea showed nitrogen fixation. Rhizobium borbori and R. rosettiformans showed a relationship with rice plants and nitrogen fixation. Among the isolates examined, AF134 and AF137 belonging to Enterobacter ludwigii and P. putida produced large amounts of IAA (92.3 μg mL⁻¹) and exhibited high nitrogenase activity (647.4 nmol C₂H₄ h⁻¹), respectively. In the plant growth test, more than 70% of the inoculated isolates showed significantly increased root and shoot dry weights. Highly diverse bacterial isolates showing promising rice growth-promoting traits were obtained from Afghanistan alkaline soils.

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 34 (4), 347-355, 2019

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• rice
• PGPR
• IAA
• ARA
• phosphate and potassium solubilization
• 16S rRNA

詳細情報

• CRID: 1390283659835484288
• NII論文ID: 130007776141
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me18168
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 030152091
• PubMed: 31527341
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030152091
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/34/4/34_ME18168/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可