Eupalinolide J induces apoptosis, cell cycle arrest, mitochondrial membrane potential disruption and DNA damage in human prostate cancer cells

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  • Wu Zeqi
    School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, China
  • Xu Xintong
    School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, China
  • Dai Lingjie
    School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, China
  • Wang Yiqi
    School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, China
  • Yang Bo
    School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, China
  • Zhao Huajun
    School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, China
  • Lou Chenghua
    School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, China

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<p>Eupalinolide J (EJ) is a new sesquiterpene lactone isolated from Eupatorium lindleyanum DC. In the present study, we investigated the anti-cancer activity of EJ on cell proliferation in human prostate cancer cells. The MTT results indicated that EJ showed marked anti-proliferative activity in PC-3 and DU-145 cells in a dose- and time-dependent manner. DAPI staining analysis demonstrated that this effect was mediated by induction of cell apoptosis. Flow cytometric analysis indicated a significant increase in apoptotic cells, cell cycle arrest at G0/G1 phase and disruption of mitochondrial membrane potential (MMP) after EJ treatment. Meanwhile, the activation of caspase-3 and caspase-9 was visibly observed. Furthermore, our results demonstrated that the expression levels of γH2AX, p-Chk1 and p-Chk2 were significantly up-regulated, suggesting the induction of DNA damage responses in EJ-treated prostate cancer cells. The above results indicated that EJ exhibited effective anti-cancer activity in vitro. It could be a promising candidate agent for the clinical treatment of prostate cancer.</p>

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