Stem blight of asparagus caused by <i>Phomopsis asparagi </i>in Myanmar and their phylogenetic grouping

DOI Open Access
  • Zaw Myo
    Institute of Tropical Agriculture, Kyushu University, Fukuoka, 812-8581, Japan
  • San Aye Seint
    Department of Plant Pathology, Yezin Agricultural University, Myanmar
  • Dihn Thao Le
    Institute of Tropical Agriculture, Kyushu University, Fukuoka, 812-8581, Japan
  • Matsumoto Masaru
    Institute of Tropical Agriculture, Kyushu University, Fukuoka, 812-8581, Japan

Abstract

<p>In 2016, stem blight disease symptoms were occurred in asparagus fields near Pyinmana, central area of Myanmar, and samples collection was conducted. The causal fungal pathogen was isolated and the representative isolates were confirmed their pathogenicity on garden asparagus (Asparagus officinalis). The morphological characteristics indicated that all isolates produced conidiomata containing alpha condinia on potato dextrose agar (PDA) media. Six randomly selected isolates were performed on phylogenetic analysis of rDNA-ITS regions, and all isolates were in a single clade with Phomopsis asparagi (GenBank accession: KJ801806 and KJ801804). The genetic diversity of 20 isolates from Myanmar and 7 isolates from Japan were also compared by using random amplified polymorphic DNA (RAPD) analysis with eight oligo primers and repetitive-element polymerase chain reaction (rep-PCR) method with BOX and ERIC primers. In both experiment, the polymorphic banding patterns of all isolates were similar and there was almost no diversity between Myanmar and Japan isolates. This is the first characterization of P. asparagi on Asparagus in Myanmar.</p>

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Details 詳細情報について

  • CRID
    1390846609799830144
  • NII Article ID
    130007790956
  • DOI
    10.11189/bita.42.23
  • ISSN
    18814212
    0915499X
  • Text Lang
    en
  • Data Source
    • JaLC
    • CiNii Articles
    • KAKEN
  • Abstract License Flag
    Disallowed

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