A Facile Assay of Epithelial-mesenchymal Transition Based on Cooperativity Quantification of Cellular Autonomous Motions

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  • YAMAMOTO Shota
    International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS)
  • MIYAMA Tatsuya
    International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS) Department of Mathematical Information Engineering, College of Industrial Technology, Nihon University
  • KOMODA Takafumi
    Department of Mechanical Engineering, Graduate School of Engineering, Chiba University
  • SUGAWARA Michiko
    Department of Mechanical Engineering, Graduate School of Engineering, Chiba University
  • NONOMURA Makiko
    Department of Mathematical Information Engineering, College of Industrial Technology, Nihon University
  • NAKANISHI Jun
    International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS) Graduate School of Advanced Science and Engineering, Waseda University

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Abstract

<p>Epithelial-mesenchymal transition (EMT), a qualitative change in cell migration behavior during cancer invasion and metastasis, is becoming a new target for anticancer drugs. Therefore, it is crucial to develop in vitro assays for the evaluation of the abilities of drug candidates to control EMT progression. We herein report on a method for the quantification of the EMT based on particle image velocimetry and correlation functions. The exponential fitting of the correlation curve gives an index (λ), which represents transforming growth factor (TGF)-β1-induced EMT progression and its suppression by inhibitors. Moreover, real-time monitoring of the λ value illustrates a time-dependent EMT progressing process, which occurs earlier than the bio-chemical changes in an EMT marker protein expression. The results demonstrate the usefulness of the present method for kinetic studies of EMT progression as well as EMT inhibitor screening.</p>

Journal

  • Analytical Sciences

    Analytical Sciences 36 (2), 263-268, 2020-02-10

    The Japan Society for Analytical Chemistry

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