A Simple Long-wavelength Fluorescent Probe for Simultaneous Discrimination of Cysteine/Homocysteine and Glutathione/Hydrogen Sulfide with Two Separated Fluorescence Emission Channels by Single Wavelength Excitation

• ZHU Hanchuang

  School of Water Conservancy and Environment, University of Jinan, Shandong Provincial Engineering Technology Research Center for Ecological Carbon Sink and Capture Utilization

• LIU Caiyun

  School of Water Conservancy and Environment, University of Jinan, Shandong Provincial Engineering Technology Research Center for Ecological Carbon Sink and Capture Utilization

• ZHANG Hanming

  School of Water Conservancy and Environment, University of Jinan, Shandong Provincial Engineering Technology Research Center for Ecological Carbon Sink and Capture Utilization

• JIA Pan

  School of Water Conservancy and Environment, University of Jinan, Shandong Provincial Engineering Technology Research Center for Ecological Carbon Sink and Capture Utilization

• LI Zilu

  School of Water Conservancy and Environment, University of Jinan, Shandong Provincial Engineering Technology Research Center for Ecological Carbon Sink and Capture Utilization

• ZHANG Xue

  School of Water Conservancy and Environment, University of Jinan, Shandong Provincial Engineering Technology Research Center for Ecological Carbon Sink and Capture Utilization

• YU Yamin

  School of Water Conservancy and Environment, University of Jinan, Shandong Provincial Engineering Technology Research Center for Ecological Carbon Sink and Capture Utilization

• SHENG Wenlong

  Biology Institute, Qilu University of Technology (Shandong Academy of Sciences)

• ZHU Baocun

  School of Water Conservancy and Environment, University of Jinan, Shandong Provincial Engineering Technology Research Center for Ecological Carbon Sink and Capture Utilization

抄録

Small molecular biothiols, such as cysteine (Cys), homocysteine (Hcy), reduced glutathione (GSH), and hydrogen sulfide (H₂S), play crucial parts in regulating the redox balance of life activities, regulating normal physiological activities and preventing various diseases. Quantitative analysis of these important small molecular substances is very important for revealing their diverse physiological and pathological effects. Although many fluorescent probes have been reported to detect biothiols in cells, it is still not sufficiently advanced to detect biothiols with separated fluorescence emission peak by same wavelength excitation. In our work, we designed a simple conjugate of Nile red and NBD (7-nitro-1,2,3-benzoxadiazole) as long-wavelength fluorescent probe NR-NBD for the simultaneous discrimination of these biothiols at single wavelength excitation. Probe NR-NBD could efficiently discriminate Cys/Hcy, GSH and H₂S by two separated fluorescence emission channels and absorption spectra. Importantly, probe NR-NBD has excellent specificity and sensitivity towards the monitoring of endogenous/exogenous Cys/Hcy and GSH/H₂S in living cells and zebrafish.

収録刊行物

• Analytical Sciences

  Analytical Sciences 36 (2), 255-262, 2020-02-10

  社団法人 日本分析化学会

キーワード

• Fluorescent probe
• two-channel imaging
• biothiol
• single wavelength excitation
• two separated fluorescence emission

詳細情報

• CRID: 1390002184873674880
• NII論文ID: 130007796071
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.19p214
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 030249704
• PubMed: 31588065
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030249704
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.19P214.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.19P214/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/36/2/36_19P214/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可