Newly isolated malic acid fermenting yeast Meyerozyma caribbica AY 33-1 for bioconversion of glucose and cassava pulp

• Thannapat Rattanapatpokin

  School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand

• Chakrit Tachaapikoon

  School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand

• Rattiya Waeonukul

  School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand

• Songsak Wattanachaisaereekul

  Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkok,Thailand

• Khanok Ratanakhanokchai

  School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand

• Patthra Pason

  School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand

抄録

This study investigated the yeast species associated with fermented foods in Thailand, focusing on identifying those that could combine the saccharification and fermentation (SSF) steps of malic acid production from cassava pulp. One hundred nighty six isolated yeast were investigated their ability to produce malic acid from glucose and cassava pulp. The result showed that only one isolate, AY33-1, proved to have amylase, xylanase and cellulase activities that crucial for cassava pulp saccharification ,which malic acid was identified as a key product for the growth on cassava pulp. Sequence analysis of the internal transcribed spacer (ITS) and 26S rDNA region indicated that the isolated strain was closely related to the species Meyerozyma caribbica with 99.8% similarity. Malic acid production from strain AY33-1 on YPD supplement with 10% glucose was 11.5 g/L and the productivity was 0.36 g/L/h. Maximum amount of malic acid production from 10% glucose was 22.7 g/L and the productivity was 0.53 g/L/h in the basal medium. The yield of malic acid was increased with 4.45 folds compared with that of Basal medium from YPD. Meyerozyma caribbica AY33-1 was the multiple enzymatic yeasts containing of 17.35U/mg amylase, 5.12 U/mg cellulase, 7.98 U/mg xylanase and also produced pullulanase when grown on cassava pulp. Our results indicate that the isolated AY33-1 is effective on direct malic acid from cassava pulp (1 g/L of malic acid), suggesting the promising yeast for developing high value-added products from cassava pulp

収録刊行物

• SEATUC journal of science and engineering

  SEATUC journal of science and engineering 1 (1), 62-70, 2020

  学校法人 芝浦工業大学

キーワード

• Malic acid
• Yeast
• Cassava pulp
• Meyerozyma caribbic. AY33-1
• Direct fermentation

詳細情報

• CRID: 1390846609805986176
• NII論文ID: 130007799441
• DOI: 10.34436/sjse.1.1_62
• ISSN: 24352993
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可