Development of 22 Microsatellite Markers for Assessing Hybridization in the Genus Gekko (Squamata: Gekkonidae)

• OKAMOTO Kota

  Graduate School of Engineering and Science, University of the Ryukyus

• KURITA Takaki

  Chiba Biodiversity Center

• NAGANO Masahiro

  Faculty of Science and Technology, Oita University

• SATO Yukuto

  Center for Strategic Research Project, Organization for Research Promotion, University of the Ryukyus

• AOYAMA Hiroaki

  Research Planning Office, Organization for Research Promotion, University of the Ryukyus

• SAITOH Seikoh

  College of Economics and Environmental Policy, Okinawa International University

• SHINZATO Naoya

  Tropical Biosphere Research Center, University of the Ryukyus

• TODA Mamoru

  Tropical Biosphere Research Center, University of the Ryukyus

書誌事項

タイトル別名

• Development of 22 Microsatellite Markers for Assessing Hybridization in the Genus <i>Gekko</i> (Squamata: Gekkonidae)

抄録

Gekko yakuensis and G. tawaensis, both endemic to western Japan, are threatened by genetic introgression from G. hokouensis and G. japonicus, respectively. To know detailed situation of their hybridizations for planning relevant conservation measures, development of sensitive genetic markers is desired. We here developed microsatellite markers based on the sequences obtained from G. hokouensis using 454 GS Junior sequencer, and tested stability of PCR amplification and species-specificity of alleles at each locus using G. hokouensis, G. yakuensis, G. japonicus, and G. tawaensis. The results showed that 22 loci were almost constantly amplified in more than one species. We further confirmed that there were fixed or nearly fixed allelic displacement between G. hokouensis and G. yakuensis, and between G. japonicus and G. tawaensis at 14 loci. Thus, these 22 loci are considered to be useful for evaluation of hybridizations between these pairs of species.

収録刊行物

• Ｃｕｒｒｅｎｔ　ｈｅｒｐｅｔｏｌｏｇｙ

  Ｃｕｒｒｅｎｔ　ｈｅｒｐｅｔｏｌｏｇｙ 39 (1), 66-74, 2020

  日本爬虫両棲類学会

キーワード

• <i>Gekko hokouensis</i>
• Hybridization
• Microsatellite
• Diagnostic markers
• Population genetics

詳細情報

• CRID: 1390002184877653120
• NII論文ID: 130007802503
• NII書誌ID: AA12246863
• DOI: 10.5358/hsj.39.66
• ISSN: 18811019
• ISSN: 13455834
• NDL書誌ID: 030277098
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030277098
• 本文言語コード: en
• データソース種別:
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