Gastrodin Inhibits H₂O₂-Induced Ferroptosis through Its Antioxidative Effect in Rat Glioma Cell Line C6

• Jiang Ting

  Key Laboratory of Xin’an Medicine, Ministry of Education, Anhui University of Chinese Medicine School of Pharmacy, Anhui University of Chinese Medicine

• Chu Jun

  Key Laboratory of Xin’an Medicine, Ministry of Education, Anhui University of Chinese Medicine

• Chen Hejuntao

  Key Laboratory of Xin’an Medicine, Ministry of Education, Anhui University of Chinese Medicine School of Pharmacy, Anhui University of Chinese Medicine

• Cheng Hui

  Key Laboratory of Xin’an Medicine, Ministry of Education, Anhui University of Chinese Medicine

• Su Jingjing

  Key Laboratory of Xin’an Medicine, Ministry of Education, Anhui University of Chinese Medicine

• Wang Xuncui

  Key Laboratory of Xin’an Medicine, Ministry of Education, Anhui University of Chinese Medicine

• Cao Yin

  Key Laboratory of Xin’an Medicine, Ministry of Education, Anhui University of Chinese Medicine

• Tian Shasha

  School of Pharmacy, Zhejiang Chinese Medical University

• Li Qinglin

  Key Laboratory of Xin’an Medicine, Ministry of Education, Anhui University of Chinese Medicine

書誌事項

タイトル別名

• Gastrodin Inhibits H₂O₂-Induced Ferroptosis through Its Antioxidative Effect in Rat Glioma Cell Line C6

抄録

Ferroptosis is a form of necrosis caused by iron-induced accumulation of lipid hydroperoxide, involving several molecular events, and has been implicated in Parkinson’s disease. Gastrodin is a component of Gastrodia elata Blume with strong antioxidant activity. We examined whether gastrodin can prevent H₂O₂-induced cytotoxicity in rat glioma cell line C6. For this purpose, C6 cells were pretreated with gastrodin (1, 5, 25 µM) and then exposed to 100 µM H₂O₂. Results showed that pretreatment of C6 cells with gastrodin decreased H₂O₂-induced lactate dehydrogenase (LDH) release and cell death. Moreover, gastrodin decreased intracellular malondialdehyde (MDA) level, whereas increased glutathione peroxidase (GPX) activity and glutathione (GSH) level after H₂O₂ treatment. In addition, treatment of deferoxamine (DFO), ferrostatin-1, and liproxstatin-1 abolished ferroptosis induced by H₂O₂ or erastin pretreatment. Treatment with gastrodin attenuated H₂O₂-induced ferroptosis and decreased lipid reactive oxygen species (ROS) (C11-BODIPY) production in C6 cells. Moreover, gastrodin increased the protein expression of nuclear factor erythroid 2-related factor 2 (Nrf2), GPX4, ferroportin-1 (FPN1), and heme oxygenase-1 (HO-1) in C6 cells treated with H₂O₂. RSL3, a GPX4 inhibitor, inhibited GPX4 protein level in cells co-treated with gastrodin and 100 µM H₂O₂. These findings indicate that gastrodin can inhibit H₂O₂-induced ferroptosis through its antioxidative effect in C6 cells.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 43 (3), 480-487, 2020-03-01

  公益社団法人 日本薬学会

キーワード

• gastrodin
• ferroptosis
• hydrogen peroxide
• neurodegenerative disease

詳細情報

• CRID: 1390565134832474240
• NII論文ID: 130007804436
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.b19-00824
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 030275039
• PubMed: 32115506
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030275039
• Web Site: https://www.jstage.jst.go.jp/article/bpb/43/3/43_b19-00824/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可