Effects of repetitive diet-induced fluctuations in plasma phosphorus on vascular calcification and inflammation in rats with early-stage chronic kidney disease

• Tani Mariko

  Graduate School of Human Science and Environment, University of Hyogo

• Tanaka Sarasa

  Graduate School of Human Science and Environment, University of Hyogo

• Takamiya Kana

  School of Human Science and Environment, University of Hyogo

• Sakaue Motoyoshi

  Graduate School of Human Science and Environment, University of Hyogo

• Ito Mikiko

  Graduate School of Human Science and Environment, University of Hyogo

Abstract

Cardiovascular disease is a major cause of death among hemodialysis patients. Hyperphosphatemia induces cardiovascular disease through vascular endothelial dysfunction and calcification. Repetition of a short-term excessive-phosphorus (P) diet causes transient elevations in plasma P and subsequent vascular endothelial dysfunction in normal rats. The purpose of this study was to investigate the effects of the P fluctuation on vascular calcification and inflammation in rats after unilateral nephrectomy as an early-stage chronic kidney disease (CKD) model. Rats were bred for 36 days; CP group, fed a control P (0.6%) diet; HP group, fed a high-P (1.2%) diet; and P fluctuation group, fed low-P (0.02%) and high-P diets alternately every 2 days. Influences on vascular calcification were analyzed using Von Kossa staining and measurement of vessel Ca content. The influence on inflammation was measured as urinary levels of 8-hydroxy-2'-deoxyguanosine. We demonstrated that the P fluctuation group showed similar vascular calcification and inflammation to the HP group, despite having the same total P intake as the CP group. A diet avoiding P fluctuations may be important for patients with early-stage CKD.

Journal

• Journal of Clinical Biochemistry and Nutrition

  Journal of Clinical Biochemistry and Nutrition 66 (2), 139-145, 2020-03-01

  SOCIETY FOR FREE RADICAL RESEARCH JAPAN

Keywords

• chronic kidney disease
• vascular calcification
• hyperphosphatemia
• oxidative stress
• phosphorus fluctuation

Details

• CRID: 1390002184878950656
• NII Article ID: 130007804503
• DOI: 10.3164/jcbn.19-93
• ISSN: 18805086
• ISSN: 09120009
• Web Site: https://www.jstage.jst.go.jp/article/jcbn/66/2/66_19-93/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2021000141
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed