Structure and diversity of 13S globulin zero-repeat subunit, the trypsin-resistant storage protein of common buckwheat (Fagopyrum esculentum M.) seeds
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- Monshi Fakhrul Islam
- Graduate School of Agriculture, Kyoto University Faculty of Agriculture, Sylhet Agricultural University
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- Khan Nadar
- Graduate School of Agriculture, Kyoto University Present address: Bio-Resources Conservation Institute, National Agricultural Research Centre (NARC)
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- Kimura Kohtaro
- Graduate School of Agriculture, Kyoto University
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- Suzuki Seita
- Graduate School of Agriculture, Kyoto University
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- Yamamoto Yuka
- Graduate School of Agriculture, Kyoto University
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- Katsube-Tanaka Tomoyuki
- Graduate School of Agriculture, Kyoto University
書誌事項
- タイトル別名
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- Structure and diversity of 13S globulin zero-repeat subunit, the trypsin-resistant storage protein of common buckwheat (<i>Fagopyrum esculentum</i> M.) seeds
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<p>The zero-repeat subunit of 13S globulin, which lacks tandem repeat inserts, is trypsin-resistant and suggested to show higher allergenicity than the other subunits in common buckwheat (Fagopyrum esculentum Moench). To evaluate allelic variations and find novel alleles, the diversity of the zero-repeat genes was examined for two Japanese elite cultivars and 15 Pakistani landraces. The results demonstrated that two new alleles GlbNA1 and GlbNC1, plus three additional new alleles GlbNA2, GlbNA3, and GlbND, were identified besides the already-known GlbNA, GlbNB, and GlbNC alleles. In the Pakistani landraces, GlbNA was the most dominant allele (0.60–0.88 of allele frequency) in all except one landrace, where GlbNB was the most dominant allele (0.50 of allele frequency). Similar to GlbNC, the alleles GlbNA2 and GlbNA3 had extra ~200 bp MITE-like sequences around the stop codon. Secondary structure predictions of a sense strand demonstrated that the extra ~200 bp sequences of GlbNC, GlbNA2, and GlbNA3 can form rigid hairpin structures with free energies of –78.95, –67.06, and –29.90 kcal/mol, respectively. These structures may affect proper transcription and/or translation. In the GlbNC homozygous line, no transcript of a zero-repeat gene was detected, suggesting the material would be useful for developing hypoallergenic buckwheat.</p>
収録刊行物
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- Breeding Science
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Breeding Science 70 (1), 118-127, 2020
日本育種学会
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詳細情報 詳細情報について
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- CRID
- 1390846609813305088
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- NII論文ID
- 130007809766
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- NII書誌ID
- AA11317194
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- ISSN
- 13473735
- 13447610
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- NDL書誌ID
- 030395498
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可