書誌事項
- タイトル別名
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- Development of tissue-specific histamine-responsive vascular endothelial models using a collagen vitrigel membrane
抄録
<p>The tightness of the endothelial barrier is tissue-dependent. We expect that tissue-specific barrier function can be induced in immature vascular endothelial cells by co-culturing with tissue-specific cells. The purpose of this study is to construct tissue-specific vascular endothelial models on a collagen vitrigel membrane (CVM) composed of high-density collagen fibrils equivalent to connective tissues in vivo and compare the responsivity to histamine. We used human microvascular endothelial cells (HMVECs) derived from a newborn foreskin. HMVECs were cultured in a CVM chamber with or without human dermal fibroblasts (HDFs), C6 cells (a rat glioma cell line) or HepG2-NIAS cells (a human hepatocellular carcinoma cell line) cultured on the reverse-side of CVM for up to 6 days. The endothelial barrier function was evaluated by transendothelial electric resistance (TEER). TEER values of a HMVEC monolayer were 15-20 Ω/cm2 during culture periods. It significantly increased up to 40-60 Ω/cm2 by co-culturing with HDFs, C6 cells and HepG2-NIAS cells. Also, TEER value was clearly decreased in the co-culture model composed of HMVECs and HDFs treated with 1 μM histamine while HMVEC monolayer model showed slight response to 100 μM histamine. Now we are investigating tissue-specific responses to histamine among the models.</p>
収録刊行物
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- 日本薬理学会年会要旨集
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日本薬理学会年会要旨集 93 (0), 1-P-077-, 2020
公益社団法人 日本薬理学会
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詳細情報 詳細情報について
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- CRID
- 1390846609812915456
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- NII論文ID
- 130007811263
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- ISSN
- 24354953
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可