アストロサイトにおけるヌクレオシド輸送体の発現と過酸化水素によるチミジン取り込み

• 田中 康一

  兵庫医療大・薬・薬理 鹿児島大・院医歯・歯科応用薬理 兵庫医科大・医・薬理

• 五十嵐 健人

  兵庫医療大・薬・薬理 鹿児島大・院医歯・歯科応用薬理

• 富田 和男

  兵庫医療大・薬・薬理 鹿児島大・院医歯・歯科応用薬理

• 北中 順惠

  兵庫医科大・医・薬理

• 北中 純一

  兵庫医科大・医・薬理

• 佐藤 友昭

  鹿児島大・院医歯・歯科応用薬理

• 竹村 基彦

  兵庫医科大・医・薬理

• 西山 信好

  兵庫医療大・薬・薬理

書誌事項

タイトル別名

• Expression of nucleoside transporters and hydrogen peroxide-induced thymidine incorporation in astrocytes

抄録

We have found that cultured differentiated astrocytes pretreated with N⁶, 2^'-O-dibutyryladenosine 3',5'-cyclic monophosphate (DBcAMP), a permeable analogue of cAMP, incorporate thymidine, but not uridine, via nucleoside transporters into TCA insoluble fraction for repair on DNA injury in the presence of hydrogen peroxide (H₂O₂) at an early time, and these phenomena are specific in differentiated astrocytes, but not undifferentiated astrocytes and neurons. We studied expression of nucleoside transporters in cultured astrocytes by RT-PCR, western blot analysis and immunocytochemistry. We could confirm CNT2, that is pyrimidine selective nucleoside transporter, CNT3, that is non-selective nucleoside transporter, ENT1, that is hypersensitive nucleoside transporter, and ENT2, that is low-sensitive nucleoside transporter, but not CNT1, that is purine selective nucleoside transporter and confirmed non-presence in brain, in cultured astrocytes. These results indicate that H₂O₂-induced thymidine incorporation could pass through specific nucleoside transporters, existed in cultured astrocytes.

収録刊行物

• 日本薬理学会年会要旨集

  日本薬理学会年会要旨集 93 (0), 3-P-277-, 2020

  公益社団法人 日本薬理学会

キーワード

• astrocyte
• transporter
• hydrogen peroxide
• expression

詳細情報

• CRID: 1390002184882602112
• NII論文ID: 130007811767
• DOI: 10.1254/jpssuppl.93.0_3-p-277
• ISSN: 24354953
• Web Site: https://www.jstage.jst.go.jp/article/jpssuppl/93/0/93_3-P-277/_pdf
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可