書誌事項
- タイトル別名
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- Inositol 1,4,5-trisphosphate receptor type 2-independent Ca<sup>2+</sup> release from the endoplasmic reticulum in astrocytes
抄録
<p>Astrocytes are actively involved in the physiological and pathophysiological functions of the brain. Ca2+ release from the endoplasmic reticulum (ER) is considered to be crucial for the regulation of astrocytic functions. Inositol 1,4,5-trisphosphate receptor type 2 (IP3R2)-knockout (KO) mice are reportedly devoid of astrocytic Ca2+ signaling, and thus widely used to explore the significance of astrocytic Ca2+ signaling. However, functional deficits in IP3R2-KO mice have been found in some reports, but not in others. To address this controversy, we re-evaluated the assumption that Ca2+ release from the ER is abolished in IP3R2-KO astrocytes. We expressed the ER luminal Ca2+ indicator G-CEPIA1er in astrocytes to directly visualize Ca2+ release from the ER. We found attenuated but significant Ca2+ release in response to application of norepinephrine to IP3R2-KO astrocytes. This IP3R2-independent Ca2+ release induced only minimal cytosolic Ca2+ transients but induced robust Ca2+ increases in mitochondria that are frequently in close contact with the ER. These results indicate that ER Ca2+ release is retained and is sufficient to increase the Ca2+ concentration in close proximity to the ER in IP3R2-KO astrocytes.</p>
収録刊行物
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- 日本薬理学会年会要旨集
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日本薬理学会年会要旨集 92 (0), 2-O-02-, 2019
公益社団法人 日本薬理学会
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詳細情報 詳細情報について
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- CRID
- 1390283659861228032
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- NII論文ID
- 130007812856
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- ISSN
- 24354953
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可