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- Mizuguchi Takaha
- Dept. Pharm., Grad. Sch. Med., Keio Univ.
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- Yasui Masato
- Dept. Pharm., Grad. Sch. Med., Keio Univ.
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- Nuriya Mutsuo
- Dept. Pharm., Grad. Sch. Med., Keio Univ.
Bibliographic Information
- Other Title
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- 第二高調波発生を用いた形質膜選択的イメージング
Abstract
<p>The plasma membrane is the site of intercellular communication and subsequent intracellular signal transduction. The specific visualization of the plasma membrane in living cells, however, is difficult using fluorescence-based techniques owing to the high background signals from intracellular organelles. In this study, we show that second harmonic generation (SHG) is a high-resolution plasma membrane-selective imaging technique that enables multifaceted investigations of the plasma membrane. In contrast to fluorescence imaging, SHG specifically visualizes the plasma membrane at locations that are not attached to artificial substrates and allows high-resolution imaging because of its subresolution nature. These properties were exploited to measure the distances from the plasma membrane to subcortical actin and tubulin fibers, revealing the precise cytoskeletal organization beneath the plasma membrane. Thus, SHG imaging enables the specific visualization of phenomena in the plasma membrane with unprecedented precision and versatility and should facilitate cell biology research focused on the plasma membrane.</p>
Journal
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- Proceedings for Annual Meeting of The Japanese Pharmacological Society
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Proceedings for Annual Meeting of The Japanese Pharmacological Society 92 (0), 1-YIA-34-, 2019
Japanese Pharmacological Society
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Keywords
Details 詳細情報について
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- CRID
- 1390002184884524928
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- NII Article ID
- 130007812910
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- ISSN
- 24354953
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed