Establishment of cardiac action potential recording using a membrane potential indicator in the mouse sinoatrial node

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  • Toyama Yuichi
    Department of Cardiology and Nephrology, Hirosaki University Graduate School of Medicine
  • Yonekura Manabu
    Department of Cardiology and Nephrology, Hirosaki University Graduate School of Medicine
  • Arakawa Yoshiaki
    Department of Pharmacology, Hirosaki University Graduate School of Medicine
  • Higuchi Shoki
    Department of Pharmacology, Hirosaki University Graduate School of Medicine
  • Tomita Hirofumi
    Department of Cardiology and Nephrology, Hirosaki University Graduate School of Medicine
  • Murakami Agnieszka M.
    Department of Pharmacology, Hirosaki University Graduate School of Medicine
  • Itagaki Shirou
    Collaboration Center for Community and Industry, Sapporo Medical University, Sapporo
  • Murakami Manabu
    Department of Pharmacology, Hirosaki University Graduate School of Medicine

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Abstract

The cardiac action potential in the sinoatrial node is important, as it propagates throughout the heart and determines cardiac rhythm. Because of the anatomical complexity, pacemaker cells in the sinoatrial node can be difficult to identify for electrophysiological analysis. Also, analysis of pacemaker cells requires a specialized technique. In this study, we tried to analyze pacemaking in the sinoatrial node. First, we evaluated the expression of various factors that participate in pacemaking or modify cardiac rhythm. Next, we evaluated recordings of the action potential in the sinoatrial node by a conventional electrophysiological technique and using oxonol V, a fluorescent voltage-sensitive dye.

Journal

  • Hirosaki Medical Journal

    Hirosaki Medical Journal 70 (2-4), 163-171, 2020

    Hirosaki University Graduate School of Medicine,Hirosaki Medical Society

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