Wound healing after applying a gelatin-based hydrogel sheet to resected rat submandibular gland
-
- Miyake Yusuke
- Division of Oral Structural and Functional Biology, Nihon University Graduate School of Dentistry
-
- Shimizu Osamu
- Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry Department of Functional Morphology, Nihon University School of Dentistry
-
- Shiratsuchi Hiroshi
- Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry Department of Functional Morphology, Nihon University School of Dentistry
-
- Tamagawa Takaaki
- Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry Department of Functional Morphology, Nihon University School of Dentistry
-
- Tonogi Morio
- Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry Department of Functional Morphology, Nihon University School of Dentistry
抄録
<p>The present study was done to develop a useful experimental model for analysis of the effects of physiologically active substances on atrophy and regeneration of salivary gland acinar cells. Resection wounds (diameter, 3 mm) were made in the submandibular glands of 8-week-old Wistar rats (n = 24) for histochemical examination on Days 3, 5, 7, 10, 14, and 21 after implantation of a gelatin-based hydrogel sheet. The results showed that the sheet had nearly disappeared by Day 10. Regions around the resection wounds were classified as normal, atrophic, or necrotic. In atrophic regions, acinar cells atrophied after resection, and few acinar cells were observed on Day 7. On Days 5-7, striated and granular ducts resembled duct-like structures. On Day 10, newly formed acinar cells were confirmed by increased periodic acid-Schiff staining, and a greater number of mature cells was present thereafter. In necrotic regions, acinar and ductal cells were destroyed, and scattered enucleated acinar cells and duct-like structures were present, on Day 3; newly formed acinar cells were observed on Day 10. Thus, the experimental model demonstrated atrophy and regeneration of the submandibular gland and enabled analysis of the effects of sustained release of physiologically active substances contained within an implanted sheet.</p>
収録刊行物
-
- Ｊｏｕｒｎａｌ ｏｆ Ｏｒａｌ Ｓｃｉｅｎｃｅ
-
Ｊｏｕｒｎａｌ ｏｆ Ｏｒａｌ Ｓｃｉｅｎｃｅ 62 (2), 222-225, 2020
日本大学歯学部