Stimulations Inducing Tethered Lipid Bilayer Formation on Protein-Modified Substrate

• Tero Ryugo

  Toyohashi University of Technology

• Sato Junichi

  Toyohashi University of Technology

Bibliographic Information

Other Title

• タンパク質修飾基板表面上で繋ぎ留め型脂質二重膜形成を誘起する刺激方法の検討
• タンパクシツ シュウショク キバン ヒョウメン ジョウ デ ツナギ トメ ガタ シシツ ニジュウマク ケイセイ オ ユウキ スル シゲキ ホウホウ ノ ケントウ

Abstract

Supported lipid bilayer (SLB) is one of artificial biomembrane systems for the studies of transportation reactions through biomembranes using solid devices and surface scientific methods. A key step in the SLB formation is transformation of spherical lipid vesicles to a planar membrane. We investigated stimulation methods for adsorbed lipid vesicles on a streptavidin-modified SiO₂/Si substrate to induce tethered-type SLB. We describe the effects of osmotic stress, Ca²⁺ addition, and a freeze-thaw cycle on the adsorbed vesicles, and on the condition of t-SLB.

Journal

• IEEJ Transactions on Electronics, Information and Systems

  IEEJ Transactions on Electronics, Information and Systems 140 (4), 447-451, 2020-04-01

  The Institute of Electrical Engineers of Japan

Keywords

• lipid bilayer membrane
• surface chemical modification
• vesicle fusion
• fluorescence microscopy

Details

• CRID: 1390002184890414080
• NII Article ID: 130007825178
• NII Book ID: AN10065950
• DOI: 10.1541/ieejeiss.140.447
• ISSN: 13488155
• ISSN: 03854221
• NDL BIB ID: 030376186
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030376186
• Web Site: https://www.jstage.jst.go.jp/article/ieejeiss/140/4/140_447/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed