Detection of antibodies against Toxoplasma gondii in cats using an immunochromatographic test based on GRA7 antigen
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- YBAÑEZ Rochelle Haidee D.
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
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- KYAN Hisako
- Okinawa Prefectural Institute of Health and Environment, 17-1 Kanekadan, Uruma-shi, Okinawa 904-2241, Japan
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- NISHIKAWA Yoshifumi
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan
書誌事項
- タイトル別名
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- Detection of antibodies against <i>Toxoplasma gondii</i> in cats using an immunochromatographic test based on GRA7 antigen
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抄録
<p>Toxoplasmosis, caused by Toxoplasma gondii, is one of the most common parasitic diseases worldwide. The GRA7 of T. gondii (TgGRA7) is an essential component of the parasitophorous vacuole (PV) and PV membrane surrounding the tachyzoites and the cyst wall of the bradyzoites. While it has been widely employed as antigen for ELISA, there is only one study that has reported its potential as antigen for immunochromatographic test (ICT) in pigs. There is no study yet documenting its potential for ICT serodiagnosis of T. gondii infection in cats. In this study, we assessed the efficacy of an ICT using TgGRA7 in the detection of Toxoplasma infections in 100 cats and compared the results with iELISAs using TgGRA7 and lysate antigens of T. gondii strains, RH, PLK, and VEG. Our results revealed that TgGRA7-ICT is a reliable test for the diagnosis of anti-T. gondii antibodies in cats, producing comparable results as conventional serological methods. This study is the first report on the use of TgGRA7 as ICT antigen for the serodiagnosis of T. gondii infection in cats.</p>
収録刊行物
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- Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ
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Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ 82 (4), 441-445, 2020
公益社団法人 日本獣医学会
