Enumeration of Chemoorganotrophic Carbonyl Sulfide (COS)-degrading Microorganisms by the Most Probable Number Method

• Kato Hiromi

  Graduate School of Life Sciences, Tohoku University

• Ogawa Takahiro

  Graduate School of Agriculture, Tokyo University of Agriculture and Technology Present address: Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology

• Ohta Hiroyuki

  Department of Bioresource Science, Ibaraki University College of Agriculture

• Katayama Yoko

  Graduate School of Agriculture, Tokyo University of Agriculture and Technology Independent Administrative Institution, Tokyo National Research Institute for Cultural Properties

Abstract

Carbonyl sulfide (COS) is the most abundant sulfur compound in the atmosphere, and, thus, is important in the global sulfur cycle. Soil is a major sink of atmospheric COS and the numerical distribution of soil microorganisms that degrade COS is indispensable for estimating the COS-degrading potential of soil. However, difficulties are associated with counting COS-degrading microorganisms using culture-dependent approaches, such as the most probable number (MPN) method, because of the chemical hydrolysis of COS by water. We herein developed a two-step MPN method for COS-degrading microorganisms: the first step for chemoorganotrophic growth that supported a sufficient number of cells for COS degradation in the second step. Our new MPN analysis of various environmental samples revealed that the cell density of COS-degrading microorganisms in forest soils ranged between 10⁶ and 10⁸ MPN (g dry soil)^–1, which was markedly higher than those in volcanic deposit and water samples, and strongly correlated with the rate of COS degradation in environmental samples. Numerically dominant COS degraders that were isolated from the MPN-positive culture were related to bacteria in the orders Bacillales and Actinomycetales. The present results provide numerical evidence for the ubiquity of COS-degrading microbes in natural environments.

Journal

• Microbes and Environments

  Microbes and Environments 35 (2), n/a-, 2020

  Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Keywords

• carbonyl sulfide
• soil microbes
• most probable number method
• carbonic anhydrase

Details

• CRID: 1390003825177655040
• NII Article ID: 130007837480
• NII Book ID: AA11551577
• DOI: 10.1264/jsme2.me19139
• ISSN: 13474405
• ISSN: 13426311
• NDL BIB ID: 030475413
• PubMed: 32350165
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030475413
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/35/2/35_ME19139/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed