Reference Intervals of Serum Non-Cholesterol Sterols by Gender in Healthy Japanese Individuals
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- Yoshida Hiroshi
- Department of Laboratory Medicine, The Jikei University Kashiwa Hospital
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- Tada Hayato
- Division of Cardiovascular and Internal Medicine, Kanazawa University Graduate School of Medical Sciences
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- Ito Kumie
- Outpatient department of Internal Medicine, Nihonbashi Sakura Clinic
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- Kishimoto Yoshimi
- Endowed Research Department Food for Health, Ochanomizu University
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- Yanai Hidekatsu
- Department of Internal Medicine, National Center for Global Health and Medicine Kohnodai Hospital
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- Okamura Tomonori
- Department of Preventive Medicine and Public Health, Keio University School of Medicine
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- Ikewaki Katsunori
- Division of Anti-aging and Vascular Medicine, Department of Internal Medicine, National Defense Medical College
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- Inagaki Kyoko
- Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Nippon Medical School
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- Shoji Tetsuo
- Department of Vascular Medicine, Graduate School of Medicine, Osaka City University
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- Bujo Hideaki
- Department of Clinical-Laboratory and Experimental-Research Medicine, Toho University Sakura Medical Center
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- Miida Takashi
- Department of Clinical Laboratory Medicine, Juntendo University School of Medicine
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- Yoshida Masayuki
- Department of Life sciences and Bioethics, Tokyo Medical and Dental University
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- Kuzuya Masafumi
- Department of Community Healthcare & Geriatrics, Nagoya University Graduate School of Medicine
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- Yamashita Shizuya
- Rinku General Medical Center, Izumisano Department of Community Medicine, Osaka University Graduate School of Medicine
抄録
<p>Aims: The present study was conducted to establish a practical method for measuring non-cholesterol sterols and reference intervals of serum levels.</p><p>Methods: Healthy subjects (109 men and 151 women), four patients with sitosterolemia, and 10 heterozygous mutation carriers of ABCG5/ABCG8 genes were investigated. Then, three non-cholesterol sterols (sitosterol, campesterol, and lathosterol) of fasting serum samples were measured via a practical and highly sensitive gas chromatography (GC) method with 0.2 µg/mL as the lower limit of quantification. The coefficient of variation (CV) values for within-run reproducibility were 3.06%, 1.89%, and 1.77% for lathosterol, campesterol, and sitosterol, respectively. The CV values for between-run reproducibility were 2.81%, 2.06%, and 2.10% for lathosterol, campesterol, and sitosterol, respectively.</p><p>Results: The serum levels of sitosterol and campesterol were significantly higher in women than in men, whereas the serum levels of lathosterol were significantly higher in men than in women. Because of these gender difference, the determination of reference intervals of the three sterol values was performed by considering gender. The reference intervals of sitosterol, campesterol, and lathosterol were 0.99–3.88, 2.14–7.43, and 0.77–3.60 µg/mL in men and 1.03–4.45, 2.19–8.34, and 0.64–2.78 µg/mL in women, respectively. The serum levels of sitosterol and campesterol were higher in patients with sitosterolemia (94.3±47.3 and 66.3±36.6 µg/mL, respectively) than in healthy subjects.</p><p>Conclusion: These results demonstrate a practical and highly sensitive GC method to measure non-cholesterol sterol levels and gender-segregated reference intervals of sitosterol, campesterol, and lathosterol in Japanese healthy subjects.</p>
収録刊行物
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- Ｊｏｕｒｎａｌ ｏｆ Ａｔｈｅｒｏｓｃｌｅｒｏｓｉｓ ａｎｄ Ｔｈｒｏｍｂｏｓｉｓ
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Ｊｏｕｒｎａｌ ｏｆ Ａｔｈｅｒｏｓｃｌｅｒｏｓｉｓ ａｎｄ Ｔｈｒｏｍｂｏｓｉｓ 27 (5), 409-417, 2020-05-01
一般社団法人 日本動脈硬化学会