Gene-expression profile reveals the genetic and acquired phenotypes of hyperactive mutant SPORTS rat

• Horiguchi Taigo

  Department of Molecular Biology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15, Kuramoto, Tokushima 770-8504, Japan

• Miyatake Yumiko

  Department of Nutrition and Metabolism, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto, Tokushima 770-8503, Japan Department of Physiology, Wakayama Medical University School of Medicine, 811-1, Kimiidera, Wakayama City, Wakayama 641-8509, Japan

• Miyoshi Keiko

  Department of Molecular Biology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15, Kuramoto, Tokushima 770-8504, Japan

• Tanimura Ayako

  Department of Molecular Biology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15, Kuramoto, Tokushima 770-8504, Japan Division of Food & Health Environmental Sciences, Department of Environmental & Symbiotic Sciences, Faculty of Environmental & Symbiotic Sciences, Prefectural University of Kumamoto, 3-1-100, Tsukide, Higashi-ku, Kumamoto 862-8502, Japan

• Hagita Hiroko

  Department of Molecular Biology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15, Kuramoto, Tokushima 770-8504, Japan

• Sakaue Hiroshi

  Department of Nutrition and Metabolism, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto, Tokushima 770-8503, Japan

• Noma Takafumi

  Department of Molecular Biology, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15, Kuramoto, Tokushima 770-8504, Japan

Abstract

Spontaneously Running Tokushima Shikoku (SPORTS) rat is a hyperactive rat strain. However, the causative mutation of this phenotype has not yet been identified. To investigate the molecular basis for the unique phenotype of SPORTS rats, we examined gene-expression profiles by microarray analyses. Among adenylate kinase isozymes that maintain the homeostasis of cellular adenine nucleotide composition in the cell, only adenylate kinase 1 is highly up-regulated in both exercised and sedentary SPORTS rats compared with wild-type (WT) rats, 5.5-fold and 3.3-fold, respectively. Further comparative analyses revealed that genes involved in glucose metabolism were up-regulated in skeletal muscle tissue of exercised SPORTS rats compared with sedentary mutants, whereas genes related to extracellular matrix or region were down-regulated compared with WT rats. In brain tissue of sedentary SPORTS rats, genes associated with defense and catecholamine metabolism were highly expressed compared with WT rats. These findings suggest that genetic mutation(s) in SPORTS rat remodels metabolic demands through differentially regulating gene expression regardless of exercise. Therefore, the SPORTS rats are useful animal model not only for further examining the effects of exercise on metabolism but also for deeply studying the molecular basis how mutation affect the psychological motivation with spontaneous voluntary exercise phenotype. J. Med. Invest. 67 : 51-61, February, 2020

Journal

• The Journal of Medical Investigation

  The Journal of Medical Investigation 67 (1.2), 51-61, 2020

  The University of Tokushima Faculty of Medicine

Keywords

• adenylate kinase
• energy metabolism
• microarray analysis
• SPORTS rats
• voluntary exercise

Details

• CRID: 1390285300155911936
• NII Article ID: 130007839959
• NII Book ID: AA12022913
• DOI: 10.2152/jmi.67.51
• ISSN: 13496867
• ISSN: 13431420
• PubMed: 32378618
• Web Site: http://repo.lib.tokushima-u.ac.jp/114556
• Web Site: https://www.jstage.jst.go.jp/article/jmi/67/1.2/67_51/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed