Characterization of Calcium Ion Release from a Polymer-Coated Electrode with a Plasticized PVC Membrane Containing Calcium Salts, and Its Contraction Examination of <i>Vorticella Convallaria</i>

• Nakamura Fumiya

  Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology

• Nakayama Taisei

  Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology

• Kageyama Itsuki

  Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology

• Kato Ryo

  Cooperative Research Facility Center, Toyohashi University of Technology

• Nagai Moeto

  Department of Mechanical Engineering, Toyohashi University of Technology

• Shibata Takayuki

  Department of Mechanical Engineering, Toyohashi University of Technology

• Sawada Kazuaki

  Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology

• Hattori Toshiaki

  Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology

抄録

Up to now several bio-imaging methods have taken very good pictures and/or recordings. With bio-imaging growing, a micro-stimulation method to limit in a fine region is required. Conventional chemical stimulation methods face difficulty in fulfilling this requirement, however, an electrochemical device is a potential response to this issue. This paper reports on the fabrication and the use of an electrochemical Ca²⁺-releasing device. The device consists of a three-layer electrode, including two transducers, a PVC membrane, PEDOT layer, and gold or carbon. The dynamic behavior of Ca²⁺ released from the device is evaluated by a two-dimensional 16 K pixel array Ca²⁺ image sensor, and is simulated using a diffusion model of Ca²⁺. Both experimental and calculated time and distance dependent profiles are almost in agreement when the PVC membrane is thin. As its biological application, a permeabilized Vorticella convallaria was set at a distance of ca. 0.1 mm from the Ca²⁺-releasing device. There was no response for some time just after switching-on, and then it gradually contracted. The gradual contraction after the interval proved that the Ca²⁺-releasing device can be used as a soft insertion based on the concentration diffusion.

収録刊行物

• Bulletin of the Chemical Society of Japan

  Bulletin of the Chemical Society of Japan 93 (5), 655-662, 2020-05-15

  公益社団法人 日本化学会

キーワード

• Polymer-coated electrode
• Plasticized PVC membrane
• Calcium ion release

詳細情報

• CRID: 1390285300157749632
• NII論文ID: 130007842977
• DOI: 10.1246/bcsj.20200015
• ISSN: 13480634
• ISSN: 00092673
• Web Site: https://academic.oup.com/bcsj/article-pdf/93/5/655/56303916/bcsj.20200015.pdf
• 本文言語コード: en
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