Inhibition Assay of Theophylline by Capillary Electrophoresis/Dynamic Frontal Analysis on the Hydrolysis of p-Nitrophenyl Phosphate with Alkaline Phosphatase

• Mine, Masanori

• Mizuguchi, Hitoshi

• Takayanagi, Toshio

抄録

A novel inhibition assay is proposed by capillary electrophoresis/dynamic frontal analysis (CE/DFA). When a substrate of p-nitrophenyl phosphate and an inhibitor of theophylline were tandemly introduced into the capillary containing alkaline phosphatase as an enzyme, two plateau signals were detected in the electropherogram. A higher plateau is based on the CE/DFA without inhibition, and a suppressed plateau is formed under the inhibition while the substrate zone passing through the inhibitor zone. Inhibition constant was successfully determined through the two plateau heights.

収録刊行物

• Chemistry Letters

  Chemistry Letters 49 (6), 681-684, 2020-03-26

  The Chemical Society of Japan

キーワード

• Capillary electrophoresis
• Alkaline phosphatase
• Inhibition assay

詳細情報

• CRID: 1050003824808280320
• NII論文ID: 130007852069
• NII書誌ID: AA12179675
• ISSN: 13480715
• ISSN: 03667022
• DOI: 10.1246/cl.200130
• Web Site: http://repo.lib.tokushima-u.ac.jp/114845
• Web Site: http://www.journal.csj.jp/doi/pdf/10.1246/cl.200130
• 本文言語コード: en
• 資料種別: journal article
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