Developmental competence of interspecies cloned embryos produced using cells from large Japanese field mice (Apodemus speciosus) and oocytes from laboratory mice (Mus musculus domesticus)

  • AZUMA Rika
    Graduate School of Biology-Oriented Science and Technology, Kindai University, Wakayama 649-6493, Japan
  • HATANAKA Yuki
    MRC Clinical Sciences Centre, Imperial College Faculty of Medicine, W12 0NN London, UK RIKEN BioResource Center, Ibaraki 305-0074, Japan
  • SHIN Seung-Wook
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Maryland 20892, USA
  • MURAI Hitoshi
    Toyama Municipal Family Park Zoo Co., Ltd., Toyama 930-0151, Japan
  • MIYASHITA Minoru
    Foundation Ube Tokiwa Zoological Society, Yamaguchi 755-0001, Japan
  • ANZAI Masayuki
    Graduate School of Biology-Oriented Science and Technology, Kindai University, Wakayama 649-6493, Japan Institute of Advanced Technology, Kindai University, Wakayama 642-0017, Japan
  • MATSUMOTO Kazuya
    Graduate School of Biology-Oriented Science and Technology, Kindai University, Wakayama 649-6493, Japan Institute of Advanced Technology, Kindai University, Wakayama 642-0017, Japan

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  • Developmental competence of interspecies cloned embryos produced using cells from large Japanese field mice (<i>Apodemus speciosus</i>) and oocytes from laboratory mice (<i>Mus musculus domesticus</i>)

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<p> The large Japanese field mouse (Apodemus speciosus) is endemic to Japan and may be used as an animal model for studies related to environmental pollution, medical science, and basic biology. However, the large Japanese field mouse has low reproductive ability due to the small number of oocytes ovulated per female. To produce experimental models, we investigated the in vitro developmental potential of interspecies somatic cell nuclear transfer (iSCNT) embryos produced by fusing tail tip cells from the large Japanese field mouse with enucleated oocytes from laboratory mice (Mus musculus domesticus). Only a small number of iSCNT embryos developed to the 4-cell (0–4%) and blastocysts (0–1%) stages under sequential treatment using trichostatin A (TSA) and vitamin C (VC) supplemented with deionized bovine serum albumin (d-BSA). This sequential treatment led to the reduction in H3K9 trimethylation and did not affect H3K4 trimethylation in at least the 2-cell stage of the iSCNT embryos. Moreover, iSCNT embryos that received tail tip cells with exposure treatment to ooplasm from cell fusion to oocyte activation or VC treatment prior to cell fusion did not exhibit significant in vitro development improvement compared to that of each control group. This suggests that large Japanese field mice/laboratory mice iSCNT embryos that received sequential treatment using TSA and VC with d-BSA may have slightly better developmental potential beyond the 4-cell stage. Our results provide insights into the reprogramming barriers impeding the wider implementation of iSCNT technology.</p>

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