Identification and analysis of a principal sigma factor interacting protein SinA, essential for growth at high temperatures in a cyanobacterium Synechococcus elongatus PCC 7942
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- Hasegawa Hazuki
- Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology School of Life Science and Technology, Tokyo Institute of Technology
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- Tsurumaki Tatsuhiro
- Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology School of Life Science and Technology, Tokyo Institute of Technology
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- Kobayashi Ikki
- Department of Applied Chemistry and Biotechnology, Chiba University
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- Imamura Sousuke
- Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology
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- Tanaka Kan
- Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology
書誌事項
- タイトル別名
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- Identification and analysis of a principal sigma factor interacting protein SinA, essential for growth at high temperatures in a cyanobacterium <i>Synechococcus elongatus</i> PCC 7942
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抄録
<p>Proteins that bind to RNA polymerase (RNAP) sigma factors play important roles in various transcriptional regulations. In this study, we identified a candidate of the principal sigma factor interacting protein in cyanobacteria, named SinA, based on a previous comprehensive protein interaction study (Sato et al., 2007) and analyzed this in the cyanobacterium Synechococcus elongatus PCC 7942. SinA is highly conserved among cyanobacteria and a knock out mutant showed defective growth at a usually permissive high temperature (40°C). Because this observation suggested SinA involvement in heat-inducible transcriptional activation, we examined heat-inducible protein gene hspA expression after temperature upshifts. The second-step induction disappeared after 15 min in the sinA mutant. In vivo pull-down experiments demonstrated the interaction between SinA and the principal sigma factor RpoD1. This SinA-RpoD1 complex was associated with an RNAP core enzyme under growth temperatures, but was dissociated after a temperature upshift. Based on these results, we propose a function of SinA to facilitate the substitution of the principal sigma factor with alternative sigma factors under heat-stressed conditions.</p>
収録刊行物
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- The Journal of General and Applied Microbiology
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The Journal of General and Applied Microbiology 66 (2), 66-72, 2020
公益財団法人 応用微生物学・分子細胞生物学研究奨励会
