Differential Localization and Invasion of Tumor Cells in Mouse Models of Human and Murine Leukemias

• Mashima Kiyomi

  Division of Hematology, Department of Medicine, Jichi Medical University

• Azuma Morio

  Division of Molecular Pharmacology, Department of Pharmacology, Jichi Medical University

• Fujiwara Ken

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University

• Inagaki Takeshi

  Division of Forensic Medicine, Department of Anatomy, Jichi Medical University

• Oh Iekuni

  Division of Hematology, Department of Medicine, Jichi Medical University

• Ikeda Takashi

  Division of Hematology, Department of Medicine, Jichi Medical University

• Umino Kento

  Division of Hematology, Department of Medicine, Jichi Medical University

• Nakano Hirofumi

  Division of Hematology, Department of Medicine, Jichi Medical University

• Morita Kaoru

  Division of Hematology, Department of Medicine, Jichi Medical University

• Sato Kazuya

  Division of Hematology, Department of Medicine, Jichi Medical University

• Minakata Daisuke

  Division of Hematology, Department of Medicine, Jichi Medical University

• Yamasaki Ryoko

  Division of Hematology, Department of Medicine, Jichi Medical University

• Ashizawa Masahiro

  Division of Hematology, Department of Medicine, Jichi Medical University

• Yamamoto Chihiro

  Division of Hematology, Department of Medicine, Jichi Medical University

• Fujiwara Shin-Ichiro

  Division of Hematology, Department of Medicine, Jichi Medical University

• Hatano Kaoru

  Division of Hematology, Department of Medicine, Jichi Medical University

• Ohmine Ken

  Division of Hematology, Department of Medicine, Jichi Medical University

• Muroi Kazuo

  Division of Hematology, Department of Medicine, Jichi Medical University

• Ohno Nobuhiko

  Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University

• Kanda Yoshinobu

  Division of Hematology, Department of Medicine, Jichi Medical University

抄録

Leukemias are refractory hematopoietic malignancies, for which the development of new therapeutic agents requires in vivo studies using tumor-bearing mouse models. Although several organs are commonly examined in such studies to evaluate the disease course, the effectiveness of interventions and the localization of tumor cells in the affected organs are still unclear. In this study, we histologically examined the distribution of leukemia cells in several organs using two leukemic mouse models produced by the administration of two cell lines (THP-1, a human myelomonocytic leukemia, and A20, a mouse B cell leukemia/lymphoma) to severe immunodeficient mice. Survival of the mice depended on the tumor burden. Although A20 and THP-1 tumor cells massively infiltrated the parenchyma of the liver and spleen at 21 days after transplantation, A20 cells were hardly found in connective tissues in Glisson’s capsule in the liver as compared with THP-1 cells. In the bone marrow, there was more severe infiltration of A20 cells than THP-1 cells. THP-1 and A20 cells were widely spread in the lungs, but were rarely observed in the small intestine. These findings suggest that each leukemia model has a unique localization of tumor cells in several affected organs, which could critically affect the disease course and the efficacy of therapeutic agents, including cellular immunotherapies.

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 53 (3), 43-53, 2020-06-26

  日本組織細胞化学会

キーワード

• leukemia
• mouse model
• immunohistochemistry
• leukemia cell line

詳細情報

• CRID: 1390566775146985856
• NII論文ID: 130007865618
• DOI: 10.1267/ahc.19035
• ISSN: 13475800
• ISSN: 00445991
• Web Site: https://www.jstage.jst.go.jp/article/ahc/53/3/53_19035/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2021123744
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可