SSR markers developed using next-generation sequencing technology in pineapple, Ananas comosus (L.) Merr

• Nashima Kenji

  College of Bioresource Sciences, Nihon University

• Hosaka Fumiko

  Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization

• Terakami Shingo

  Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization

• Kunihisa Miyuki

  Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization

• Nishitani Chikako

  Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization

• Moromizato Chie

  Okinawa Prefectural Agricultural Research Center Nago Branch

• Takeuchi Makoto

  Okinawa Prefectural Agricultural Research Center Nago Branch

• Shoda Moriyuki

  Okinawa Prefectural Agricultural Research Center

• Tarora Kazuhiko

  Okinawa Prefectural Agricultural Research Center

• Urasaki Naoya

  Okinawa Prefectural Agricultural Research Center

• Yamamoto Toshiya

  Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization

書誌事項

タイトル別名

• SSR markers developed using next-generation sequencing technology in pineapple, <i>Ananas comosus</i> (L.) Merr.

抄録

Simple sequence repeat (SSR) markers provide a reliable tool for the identification of accessions and the construction of genetic linkage maps because of their co-dominant inheritance. In the present study, we developed new SSR markers with next-generation sequencing using the Roche 454 GS FLX+ platform. Five hundred SSR primer sets were tested for the genetic identification of pineapple, including 100 each for the di-, tri-, tetra-, penta-, and hexa-nucleotide motif SSRs. In total, 160 SSR markers successfully amplified fragments and exhibited polymorphism among accessions. The SSR markers revealed the number of alleles per locus (ranging from 2 to 13), the expected heterozygosity (ranging from 0.041 to 0.823), and the observed heterozygosity (ranging from 0 to 0.875). A total of 117 SSR markers with tri- or greater nucleotide motifs were shown to be effective at facilitating accurate genotyping. Using the SSR markers, 25 accessions were distinguished genetically, with the exception of accessions ‘MD-2’ and ‘Yonekura’. The developed SSR markers could facilitate the establishment of efficient and accurate genetic identification systems and the construction of genetic linkage maps in the future.

収録刊行物

• Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ

  Ｂｒｅｅｄｉｎｇ　Ｓｃｉｅｎｃｅ 70 (3), 415-421, 2020

  日本育種学会

キーワード

• <i>Ananas comosus</i>
• NGS
• SSR marker
• identification of accessions

詳細情報

• CRID: 1390285300170828032
• NII論文ID: 130007866649
• NII書誌ID: AA11317194
• DOI: 10.1270/jsbbs.19158
• ISSN: 13473735
• ISSN: 13447610
• NDL書誌ID: 030541565
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I030541565
• Web Site: https://www.jstage.jst.go.jp/article/jsbbs/70/3/70_19158/_pdf
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
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